弓形虫感染对子一代雄性小鼠脑部多巴胺水平的影响
发布时间：2019-06-03 21:47
【摘要】：目的探讨弓形虫感染对子一代雄性小鼠发育期脑部多巴胺水平的影响。方法 36只雌性ICR小鼠随机分为健康对照组和弓形虫感染组,每组18只。感染组每鼠分别经口感染弓形虫PRU弱毒株10个包囊。感染后90 d,与健康雄性ICR小鼠按1∶1交配。每组各取2只交配成功的母鼠于孕20 d时剖腹取胎鼠。其他交配成功的母鼠自然分娩。应用高效液相色谱-电化学法对孕20 d的雄性胎鼠及出生后14 d和63 d的雄性仔鼠(各6只)进行脑皮层、小脑、海马和纹状体多巴胺含量的测定。结果感染组小鼠感染后死亡3只,余15只感染鼠中,仅6只交配成功。2只于孕20 d剖腹取胎鼠12只,其中雄性7只;4只自然分娩,仔鼠存活21只,其中雄性15只。18只对照组小鼠均交配成功,2只于孕20 d时剖腹取胎鼠23只,其中雄性12只;16只自然分娩,仔鼠存活179只,其中雄性92只。感染组和对照组雄性胎鼠小脑区多巴胺含量分别为(413.25±21.78)ng/g和(346.30±51.83)ng/g,感染组显著高于对照组(P0.01),两者皮层区多巴胺含量差异无统计学意义(P0.05)。感染组出生后14 d和63 d,雄性仔鼠皮层区[(462.50±24.80)ng/g和(1 215.77±113.64)ng/g]、小脑区[(271.55±26.19)ng/g和(1 328.82±39.62)ng/g]、海马区[(225.78±24.17)ng/g和(1 322.70±58.34)ng/g]和纹状体区[(455.23±61.53)ng/g和(991.32±54.31)ng/g]的多巴胺含量均显著高于相应对照组(P0.05或P0.01)。结论弓形虫感染能引起子一代雄性小鼠发育期脑部多巴胺水平显著升高。
[Abstract]:Objective to investigate the effect of Toxoplasma gondii (Toxoplasma gondii) infection on dopamine levels in the brain of first generation male mice during development. Methods 36 female ICR mice were randomly divided into healthy control group and Toxoplasma gondii infection group with 18 mice in each group. In the infection group, each mouse was infected with 10 cysts of Toxoplasma gondii PRU attenuated strain. 90 days after infection, the mice were mated with healthy male ICR mice at 1:1. In each group, 2 successfully mated female mice were taken by caesarean section on the 20th day of pregnancy. Other successfully mated female mice give birth naturally. The contents of dopamine in cerebral cortex, cerebellum, hippocampus and striatum of male fetal rats on the 20th day of gestation and 6 on the 14th and 63rd day after birth were determined by high performance liquid chromatography-electrochemical method. Results in the infection group, 3 mice died after infection, and only 6 of the remaining 15 infected mice mated successfully. 12 fetal mice, including 7 males, were taken by laparotomy on the 20th day of gestation. Four mice gave birth naturally, 21 of them survived, of which 15 were males. All the 18 mice in the control group mated successfully, 23 of them were taken by caesarean section on the 20th day of gestation, of which 12 were males, and 179 of them survived naturally, of which 92 were males. The dopamine content in cerebellar area of male fetal rats in infection group and control group was (413.25 卤21.78) ng/g and (346.30 卤51.83) ng/g, respectively, which was significantly higher than that in control group (P 0.01). There was no significant difference in dopamine content between the two groups (P 0.05). In the infection group, 14 and 63 days after birth, the cortical area of male offspring [(462.50 卤24.80) ng/g and (1 215.77 卤113.64) ng/g], cerebellar area [(271.55 卤26.19) ng/g and (1 328.82 卤39.62) ng/g], The dopamine contents in hippocampus [(225.78 卤24.17) ng/g and (1 322.70 卤58.34) ng/g] and striatum [(455.23 卤61.53) ng/g and (991.32 卤54.31) ng/g] were significantly higher than those in the corresponding control. Group P (P 0.05 or P 0.01). Conclusion Toxoplasma gondii infection can significantly increase the level of dopamine in the brain of male mice during development.
【作者单位】： 江苏省寄生虫病防治研究所
【基金】：“十一五”国家科技支撑计划重点项目(No.2010BAD04B02) 教育部创新团队发展计划(No.IRT0978)~~
【分类号】：R531.8
[Abstract]:Objective to investigate the effect of Toxoplasma gondii (Toxoplasma gondii) infection on dopamine levels in the brain of first generation male mice during development. Methods 36 female ICR mice were randomly divided into healthy control group and Toxoplasma gondii infection group with 18 mice in each group. In the infection group, each mouse was infected with 10 cysts of Toxoplasma gondii PRU attenuated strain. 90 days after infection, the mice were mated with healthy male ICR mice at 1:1. In each group, 2 successfully mated female mice were taken by caesarean section on the 20th day of pregnancy. Other successfully mated female mice give birth naturally. The contents of dopamine in cerebral cortex, cerebellum, hippocampus and striatum of male fetal rats on the 20th day of gestation and 6 on the 14th and 63rd day after birth were determined by high performance liquid chromatography-electrochemical method. Results in the infection group, 3 mice died after infection, and only 6 of the remaining 15 infected mice mated successfully. 12 fetal mice, including 7 males, were taken by laparotomy on the 20th day of gestation. Four mice gave birth naturally, 21 of them survived, of which 15 were males. All the 18 mice in the control group mated successfully, 23 of them were taken by caesarean section on the 20th day of gestation, of which 12 were males, and 179 of them survived naturally, of which 92 were males. The dopamine content in cerebellar area of male fetal rats in infection group and control group was (413.25 卤21.78) ng/g and (346.30 卤51.83) ng/g, respectively, which was significantly higher than that in control group (P 0.01). There was no significant difference in dopamine content between the two groups (P 0.05). In the infection group, 14 and 63 days after birth, the cortical area of male offspring [(462.50 卤24.80) ng/g and (1 215.77 卤113.64) ng/g], cerebellar area [(271.55 卤26.19) ng/g and (1 328.82 卤39.62) ng/g], The dopamine contents in hippocampus [(225.78 卤24.17) ng/g and (1 322.70 卤58.34) ng/g] and striatum [(455.23 卤61.53) ng/g and (991.32 卤54.31) ng/g] were significantly higher than those in the corresponding control. Group P (P 0.05 or P 0.01). Conclusion Toxoplasma gondii infection can significantly increase the level of dopamine in the brain of male mice during development.
【作者单位】： 江苏省寄生虫病防治研究所
【基金】：“十一五”国家科技支撑计划重点项目(No.2010BAD04B02) 教育部创新团队发展计划(No.IRT0978)~~
【分类号】：R531.8
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