CD46、Nectin-4在麻疹病毒感染人肺泡上皮细胞中的作用

发布时间：2019-06-04 08:38

【摘要】：目的:探讨CD46、Nectin-4在麻疹病毒感染人肺泡上皮细胞中的作用。以及CD46和Nectin-4之间的相互作用。方法:分为麻疹病毒感染前处理组和感染后2 h组,均用抗CD46抗体、抗Nectin-4抗体处理人肺泡上皮细胞,并设对照组。检测细胞内病毒滴度的变化,采用Western blot和Real-time PCR检测NP蛋白的表达水平,并运用免疫共沉淀检测CD46与Nectin-4的相互作用。结果:与对照组相比,麻疹病毒感染前用抗CD46抗体、抗Nectin-4抗体处理的人肺泡上皮细胞内麻疹病毒滴度显著降低,分别为对照组的48.03%和49.53%,同时用抗CD46抗体、抗Nectin-4抗体处理使病毒滴度下降为27.15%,差异均有统计学意义(P0.01)。然而,在麻疹病毒感染2 h后用抗CD46抗体、抗Nectin-4抗体处理,人肺泡上皮细胞内麻疹病毒滴度和NP蛋白量未见显著减少。Western blot和Real-time PCR结果与上述结果一致;免疫共沉淀实验显示,CD46与Nectin-4存在相互作用。结论:CD46、Nectin-4在麻疹病毒感染肺泡上皮细胞中起到介导作用,CD46与Nectin-4可能存在协同作用,该作用对麻疹病毒的感染起到了增强效果。
[Abstract]:Objective: to investigate the role of CD46,Nectin-4 in human alveolar epithelial cells infected with measles virus. And the interaction between CD46 and Nectin-4. Methods: human alveolar epithelial cells were treated with anti-CD46 antibody and anti-Nectin-4 antibody, and the control group was set up. The changes of virus titer in cells were detected, the expression of NP protein was detected by Western blot and Real-time PCR, and the interaction between CD46 and Nectin-4 was detected by immunoprecipitation. Results: compared with the control group, the titer of measles virus in human alveolar epithelial cells treated with anti-CD46 antibody and anti-Nectin-4 antibody was significantly lower than that in the control group (48.03% and 49.53%, respectively). At the same time, anti-CD46 antibody was used at the same time. Anti-Nectin-4 antibody treatment decreased the virus titer to 27.15%, the difference was statistically significant (P 0.01). However, after 2 hours of measles virus infection, the titer of measles virus and the content of NP protein in human alveolar epithelial cells were not significantly decreased when treated with anti-CD46 antibody and anti-Nectin-4 antibody. Western blot and Real-time PCR results were consistent with the above results. Immunoprecipitation assay showed that there was interaction between CD46 and Nectin-4. Conclusion: CD46,Nectin-4 plays a mediating role in the infection of alveolar epithelial cells by measles virus. There may be a synergistic effect between CD46 and Nectin-4, which enhances the infection of measles virus.
【作者单位】：湖北省通城县人民医院;湖北文理学院医学院;
【基金】：湖北省卫生与计划生育委员会项目(No.WJ2015MB301)
【分类号】：R511.1

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