日本血吸虫尾蚴逸出节律性及尾蚴趋向吸附的研究
发布时间：2019-06-14 10:03
【摘要】：第一部分日本血吸虫尾蚴逸出昼夜节律性及光照周期变化对其影响 目的:观察感染性钉螺体内日本血吸虫尾蚴逸出的昼夜节律性，以及光照周期变化对此特征的影响。 方法:以安徽（山区）和江苏（湖区）两地感染性钉螺为研究对象，分别将钉螺置于自然光照、昼夜颠倒、7:00～9:00插入短暂黑暗、17:00～19:00插入短暂黑暗这四种光照周期条件下连续逸蚴24h，记录每2h时间段内感染性钉螺体内日本血吸虫尾蚴逸出数量，，从而探讨光照周期变化与尾蚴逸出的关系。 结果：在自然光照周期下，山区感染性钉螺体内日本血吸虫尾蚴逸出高峰时段为下午17:00～21:00，表现为‘晚逸蚴’；湖区感染性钉螺体内日本血吸虫尾蚴逸出高峰时段为上午7:00～11:00，表现为‘早逸蚴’。在昼夜颠倒的光照周期下，湖区感染性钉螺体内日本血吸虫尾蚴逸出节律性出现逆转；上午7:00～9:00插入短暂黑暗会相应延迟山区和湖区感染性钉螺体内日本血吸虫尾蚴逸出高峰时段；下午17:00～19:00插入短暂黑暗对两地感染性钉螺体内日本血吸虫尾蚴逸出节律性几乎没有影响。 结论：日本血吸虫尾蚴逸出呈现昼夜节律性，光照周期变化会相应改变尾蚴逸出节律性。 第二部分日本血吸虫尾蚴的趋向吸附作用以及温度和尾蚴时龄对其影响 目的：观察日本血吸虫尾蚴对油酸的趋向吸附能力，以及尾蚴时龄和水温对此特征的影响。 方法：利用蜂蜡、指甲油的可凝固性与不同浓度（0.15g/ml、0.3g/ml、0.6g/ml）油酸混合后制备成一种皮肤模拟物，供尾蚴吸附。空白组、蜂蜡组、指甲油组作为对照组。模拟小鼠腹部贴片感染尾蚴的方法，用接种环挑取50±5条尾蚴，置于预先制备好的皮肤模拟物上，一段时间后滴加卢戈氏碘液固定染色，显微镜下计算皮肤模拟物上尾蚴断尾率。或揭去盖玻片，用去氯水多次冲洗皮肤模拟物后滴加卢戈氏碘液固定染色，显微镜下计数经去氯水冲洗后仍吸附在皮肤模拟物上的尾蚴数量。 结果：油酸组皮肤模拟物上尾蚴断尾率及吸附数量与对照组相比较存在极其显著性差异（P＜0.01）。尾蚴与皮肤模拟物接触时间相等时，0.15g/ml油酸组与0.3g/ml油酸组皮肤模拟物上尾蚴断尾率存在显著性差异（P＜0.05），0.3g/ml油酸组与0.6g/ml油酸组皮肤模拟物上尾蚴断尾率不存在显著性差异。油酸浓度相同时，逸出后0h尾蚴与逸出后5h尾蚴在皮肤模拟物上吸附数量无显著性差异；逸出后5h尾蚴与逸出后10h尾蚴在皮肤模拟物上吸附数量存在显著性差异（P＜0.05）。水温变化（25～35℃）对尾蚴断尾率及吸附数量均无显著性差异。 结论：日本血吸虫尾蚴对油酸具有趋向吸附作用，尾蚴时龄影响尾蚴趋向吸附的能力。水温变化（25～35℃）对其影响不大。
[Abstract]:The effect of the circadian rhythm of the first part of Schistosoma japonicum and the changes of the photoperiod Objective: To observe the circadian rhythm of the tail of Schistosoma japonicum in the infected Oncomelania hupensis and the shadow of the changes in the photoperiod. In this paper, Oncomelania hupensis (Oncomelania hupensis) in Anhui (mountain area) and Jiangsu (lake area) were used as the research object, and the snail was put into the natural light, the day and night was reversed,7:00 ~ 9:00 was inserted into the short dark, and 17:00 ~ 19:00 was inserted into the short dark. After 24 h, the amount of the tail of Schistosoma japonicum in the infected snails was recorded for every 2 h, so as to study the changes of the illumination period and the escape of the tail. The results showed that during the natural light period, the peak period of the tail of Schistosoma japonicum in the infected snails was 17:00 to 21:00 in the afternoon, and the peak period was 7:00 a.m. to 7:00 AM in the infected Oncomelania hupensis. 11:00, performance as In the period of day and night, the occurrence of the rhythm of the tail of Schistosoma japonicum in the infected Oncomelania hupensis in the lake area is reversed; the insertion of the short darkness at 7:00 to 9:00 in the morning will delay the peak period of the tail of the Schistosoma japonicum in the infected snails in the mountain area and the lake area; and 17: 00-19:00 insertion of short-time dark on the rhythm of the tail of Schistosoma japonicum in the infected snails in the two places There is no effect. Conclusion: The circadian rhythm of the tail of Schistosoma japonicum and the change of light cycle can be changed accordingly. The rhythm of the tail of the tail, the tendency of the second part of the tail of the Schistosoma japonicum, and the temperature. Objective: To observe the tendency of the tail of Schistosoma japonicum to the tendency of oleic acid to adsorb and the tail of the tail. The effect of age and water temperature on this characteristic was studied. The method was prepared by mixing the setting of beeswax and nail polish with different concentration (0.15 g/ ml, 0.3 g/ ml, 0.6 g/ ml) of oleic acid. The invention relates to a skin mimetic, which is used for the absorption of the tail gas. The blank group Cera Flava and nail polish group were used as control group. The method for simulating the infection of the tail of the abdominal patch of mice was carried out by using the inoculation ring to pick up 50% of the tail of the tail of the skin, and then put it on the pre-prepared skin dummy. After a period of time, the iodine solution of Lugol's iodine was added for fixation, and the microscope was under the microscope. The tail-tail rate of the skin-simulating object is calculated, or the cover glass is removed, and after the skin simulation is washed with the dechlorine water for several times, the lugohlet iodine solution is added for fixing and dyeing, the under-microscope counting is still absorbed after the chlorine-removing water is washed, The number of tails attached to the skin mimetics. Results: The tail and tail and the number of adsorption on the skin of the oleic acid group were compared with the control group. There was a significant difference (P <0.01). When the contact time of the tail hair and the skin analog was the same, there was a significant difference (P <0.05), 0.3 g/ ml of oleic acid group and 0.6 g/ ml of the skin of the oleic acid group. There was no significant difference in the tail-tail rate of the tail of the skin. When the concentration of the oleic acid was the same, there was no significant difference in the number of adsorption of the tail of the tail of the tail of the tail of the tail after the escape and the 5-hour after the escape. There was a significant difference (P <0.05). The change of water temperature (25 ~ 35 鈩
本文编号：2499309
[Abstract]:The effect of the circadian rhythm of the first part of Schistosoma japonicum and the changes of the photoperiod Objective: To observe the circadian rhythm of the tail of Schistosoma japonicum in the infected Oncomelania hupensis and the shadow of the changes in the photoperiod. In this paper, Oncomelania hupensis (Oncomelania hupensis) in Anhui (mountain area) and Jiangsu (lake area) were used as the research object, and the snail was put into the natural light, the day and night was reversed,7:00 ~ 9:00 was inserted into the short dark, and 17:00 ~ 19:00 was inserted into the short dark. After 24 h, the amount of the tail of Schistosoma japonicum in the infected snails was recorded for every 2 h, so as to study the changes of the illumination period and the escape of the tail. The results showed that during the natural light period, the peak period of the tail of Schistosoma japonicum in the infected snails was 17:00 to 21:00 in the afternoon, and the peak period was 7:00 a.m. to 7:00 AM in the infected Oncomelania hupensis. 11:00, performance as In the period of day and night, the occurrence of the rhythm of the tail of Schistosoma japonicum in the infected Oncomelania hupensis in the lake area is reversed; the insertion of the short darkness at 7:00 to 9:00 in the morning will delay the peak period of the tail of the Schistosoma japonicum in the infected snails in the mountain area and the lake area; and 17: 00-19:00 insertion of short-time dark on the rhythm of the tail of Schistosoma japonicum in the infected snails in the two places There is no effect. Conclusion: The circadian rhythm of the tail of Schistosoma japonicum and the change of light cycle can be changed accordingly. The rhythm of the tail of the tail, the tendency of the second part of the tail of the Schistosoma japonicum, and the temperature. Objective: To observe the tendency of the tail of Schistosoma japonicum to the tendency of oleic acid to adsorb and the tail of the tail. The effect of age and water temperature on this characteristic was studied. The method was prepared by mixing the setting of beeswax and nail polish with different concentration (0.15 g/ ml, 0.3 g/ ml, 0.6 g/ ml) of oleic acid. The invention relates to a skin mimetic, which is used for the absorption of the tail gas. The blank group Cera Flava and nail polish group were used as control group. The method for simulating the infection of the tail of the abdominal patch of mice was carried out by using the inoculation ring to pick up 50% of the tail of the tail of the skin, and then put it on the pre-prepared skin dummy. After a period of time, the iodine solution of Lugol's iodine was added for fixation, and the microscope was under the microscope. The tail-tail rate of the skin-simulating object is calculated, or the cover glass is removed, and after the skin simulation is washed with the dechlorine water for several times, the lugohlet iodine solution is added for fixing and dyeing, the under-microscope counting is still absorbed after the chlorine-removing water is washed, The number of tails attached to the skin mimetics. Results: The tail and tail and the number of adsorption on the skin of the oleic acid group were compared with the control group. There was a significant difference (P <0.01). When the contact time of the tail hair and the skin analog was the same, there was a significant difference (P <0.05), 0.3 g/ ml of oleic acid group and 0.6 g/ ml of the skin of the oleic acid group. There was no significant difference in the tail-tail rate of the tail of the skin. When the concentration of the oleic acid was the same, there was no significant difference in the number of adsorption of the tail of the tail of the tail of the tail of the tail after the escape and the 5-hour after the escape. There was a significant difference (P <0.05). The change of water temperature (25 ~ 35 鈩
本文编号：2499309
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