3种结核病快速诊断新技术与传统方法的对比分析
发布时间：2019-08-02 21:47
【摘要】：目的探讨3种诊断技术对结核病及耐药结核病的应用价值。方法使用LED法、Xpert MTB/RIF、固体培养检测259例疑似结核病患者痰标本,比较各方法的阳性率、灵敏度与特异度;142份菌株使用比例法、Xpert RIF与线性探针检测利福平耐药,比较2种方法与比例法的差异。结果 259例痰标本中,固体培养、LED法、Xpert MTB阳性率分别为54.83%、44.79%、55.60%,平均时间为32.6 d、0.5 h、2.5 h;以固体培养阳性的142例为金标准,LED法、Xpert MTB阳性数分别为109例、129例,灵敏度为76.76%、90.85%,特异度为94.02%、87.18%。对142例菌株进行药敏试验,利福平耐药菌株34例,时间为28 d;以34例为金标准,Xpert RIF、线性探针检测利福平耐药符合率分别为94.12%、91.18%,时间为2.5 h、8 h。结论 3种检测技术对结核病及耐药结核病的诊断具有灵敏性高、特异性高、检测时间短等特点,在临床中有很好的应用前景与推广价值。
[Abstract]:Objective to evaluate the value of three diagnostic techniques in tuberculosis and drug-resistant tuberculosis. Methods sputum samples from 259 suspected tuberculosis patients were detected by LED and Xpert MTB/RIF, solid culture, the positive rate, sensitivity and specificity of each method were compared, and rifampicin resistance was detected by proportional, Xpert RIF and linear probe in 142 strains, and the difference between the two methods and the proportional method was compared. Results in 259 sputum samples, the positive rate of, Xpert MTB by LED method was 54.83%, 44.79%, 55. 60%, the average time was 32. 6 days, 0.5 h, 2.5 h, and the positive number of, Xpert MTB by solid culture was 109 cases, 129 cases, sensitivity was 76. 76%, 90. 85%, specificity was 94. 02%, 87. 18%, the positive rate of, Xpert MTB was 76. 76%, 90. 85%, the specificity was 94. 02%, the specificity was 87. 18%, the positive rate of Led was 76. 76%, 90. 85%, the specificity was 94. 02%, the specificity was 87. 18%. The drug sensitivity test was carried out in 34 cases of rifampicin resistant strains for 28 days, and the coincidence rate of rifampicin resistance detected by gold standard, Xpert RIF, linear probe in 34 cases was 94.12%, 91.8%, and the time was 2.5 h and 8 h, respectively. the coincidence rate of rifampicin resistance was 94.12% and 41.18%, respectively. Conclusion the three detection techniques have the characteristics of high sensitivity, high specificity and short detection time in the diagnosis of tuberculosis and drug-resistant tuberculosis, and have a good application prospect and popularization value in clinic.
【作者单位】： 阜阳市疾病预防控制中心;
【分类号】：R52
，
本文编号：2522375
[Abstract]:Objective to evaluate the value of three diagnostic techniques in tuberculosis and drug-resistant tuberculosis. Methods sputum samples from 259 suspected tuberculosis patients were detected by LED and Xpert MTB/RIF, solid culture, the positive rate, sensitivity and specificity of each method were compared, and rifampicin resistance was detected by proportional, Xpert RIF and linear probe in 142 strains, and the difference between the two methods and the proportional method was compared. Results in 259 sputum samples, the positive rate of, Xpert MTB by LED method was 54.83%, 44.79%, 55. 60%, the average time was 32. 6 days, 0.5 h, 2.5 h, and the positive number of, Xpert MTB by solid culture was 109 cases, 129 cases, sensitivity was 76. 76%, 90. 85%, specificity was 94. 02%, 87. 18%, the positive rate of, Xpert MTB was 76. 76%, 90. 85%, the specificity was 94. 02%, the specificity was 87. 18%, the positive rate of Led was 76. 76%, 90. 85%, the specificity was 94. 02%, the specificity was 87. 18%. The drug sensitivity test was carried out in 34 cases of rifampicin resistant strains for 28 days, and the coincidence rate of rifampicin resistance detected by gold standard, Xpert RIF, linear probe in 34 cases was 94.12%, 91.8%, and the time was 2.5 h and 8 h, respectively. the coincidence rate of rifampicin resistance was 94.12% and 41.18%, respectively. Conclusion the three detection techniques have the characteristics of high sensitivity, high specificity and short detection time in the diagnosis of tuberculosis and drug-resistant tuberculosis, and have a good application prospect and popularization value in clinic.
【作者单位】： 阜阳市疾病预防控制中心;
【分类号】：R52
，
本文编号：2522375
本文链接：https://www.wllwen.com/yixuelunwen/chuanranbingxuelunwen/2522375.html
最近更新
教材专著
