采用四代试剂结合核酸检测方法早期发现HIV感染者的检测策略

发布时间：2019-08-26 11:48

【摘要】：目的在用艾滋病病毒(HIV)抗体诊断试剂盒(简称第三代试剂)筛查的基础上,采用HIV抗原抗体(HIV Ag/Ab)诊断试剂(简称第四代试剂)结合核酸检测方法,对天津男男性行为人群(MSM)筛查HIV结果进行评价,建立发现筛查阳性感染者的策略。方法对天津市2013年MSM的血液样品2320例,采用第三代试剂进行初筛,对筛查阴性的样品采用第四代试剂与核酸检测进一步筛查。结果2320例样品中,仅用第三代试剂检出HIV抗体阳140例,加用第4代试剂检出阳性146例,再加用核酸方法检出阳性151例,第四代试剂结合核酸的方法比只采用第三代试剂的方法,多检出11例早期感染的阳性样品,阳性率从6.03%提高至6.51%。结论在感染率较高的MSM中,采用第四代试剂结合集合核酸方法,可以提高HIV感染者的检出率,最大限度地发现早期感染者,减少二代传播,建议将第四代试剂作为高危人群HIV筛查的首选试剂。
[Abstract]:Objective on the basis of screening with (HIV) antibody diagnostic kit (the third generation reagent), HIV antigen antibody (HIV Ag/Ab) diagnostic reagent (fourth generation reagent) combined with nucleic acid detection method was used to evaluate the results of (MSM) screening HIV in Tianjin male sex population, and to establish a strategy for finding positive infection. Methods 2320 blood samples of MSM in Tianjin in 2013 were screened by the third generation reagent, and the fourth generation reagent and nucleic acid test were used to further screen the negative samples. Results among 2320 samples, only 140cases of positive HIV antibody were detected by the third generation reagent, 146cases were positive by adding the fourth generation reagent, and 151cases were positive by nucleic acid method. The positive rate of the fourth generation reagent combined with nucleic acid was 6.51% higher than that of the third generation reagent alone, and the positive rate of the fourth generation reagent combined with nucleic acid was 6.51% higher than that of the third generation reagent. Conclusion in MSM with high infection rate, the detection rate of HIV infection can be improved, the early infection rate can be detected to the maximum extent, and the second generation transmission can be reduced by using the fourth generation reagent combined with collective nucleic acid method. It is suggested that the fourth generation reagent should be used as the first choice for HIV screening in high risk population.
【作者单位】：天津市疾病预防控制中心;天津市红桥区疾病预防控制中心;天津市卫生局;
【基金】：天津市卫生行业重点攻关项目(12KG129,14KG119)~~
【分类号】：R512.91

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