干扰素诱导跨膜蛋白3 rs12252单核苷酸多态性与乙型流感临床严重程度的相关性研究

发布时间：2019-08-24 10:46

【摘要】：为了研究干扰素诱导的跨膜蛋白3(Interferon-induced transmembrane protein 3,IFITM 3)rs12252单核苷酸多态性(Single nucleotide polymorphism,SNP)与乙型流感临床严重程度的相关性,在吉林省和湖南省收集了181名乙型流感轻症病例和83名乙型流感重症病例全血标本,提取两组病例基因组DNA并扩增相应的目的片段后,采用Sanger测序的方法对ITIM3rs12252SNP进行基因分型。同时选用千人基因组103名中国北京地区汉族人(CHB)为一般人群对照。统计分析结果表明,轻症病例IFITM 3rs12252基因型频率分布与一般人群对照无显著性差异,但重症病例rs12252CC基因型频率显著高于一般人群对照组。乙型流感重症病例IFITM3rs12252C等位基因频率显著高于轻症病例(P0.05),携带rs12252-C等位基因发展为乙型流感重症的风险是rs12252-T等位基因的1.818倍(Odds Ratio=1.818,95%CI:1.241~2.664)。并且重症病例中rs12252CC基因型频率显著高于轻症病例(P0.05),表明rs12252CC基因型与乙型流感重症相关。
[Abstract]:In order to study the correlation between the polymorphism of the interferon-induced transmembrane protein 3 (IFITM 3) rs12252 and the clinical severity of influenza B, In Jilin province and Hunan province,181 cases of influenza B and 83 of the whole blood of the severe case of influenza B were collected. The genomic DNA of the two groups was extracted and the corresponding target fragment was amplified, and the gene of the ITIM3rs12252SNP was genotyped by using the method of Sanger sequencing. At the same time,103 Chinese Han people (CHB) were selected as the control of the general population. The results of the statistical analysis showed that the frequency distribution of the IITM 3 rs12252 genotype was not significantly different from that of the control group in the general population, but the frequency of rs12252CC in the severe case was significantly higher than that of the control group in the general population. The frequency of the IFITMrs12252C allele in the severe case of influenza B was significantly higher than that of the mild case (P0.05). The risk of the development of the rs12252-C allele was 1.818 times that of the rs12252-T allele (Ods Ratio = 1.818,95% CI: 1.241-2.664). The genotype frequency of rs12252CC was significantly higher in the severe cases (P0.05), indicating that the rs12252CC genotype was related to the severe type of influenza B.
【作者单位】：中国疾病预防控制中心病毒病预防控制所世界卫生组织全球流感参比和研究合作中心感染性疾病诊治协同创新中心卫生和计划生育委员会医学病毒和病毒病重点实验室;湖南省疾病预防控制中心;吉林省疾病预防控制中心;
【基金】：国家重点研发计划(项目号:2016YFC1200201),题目:新发突发病原体发生和演化过程中的关键调频分子及其机制研究国家杰出青年科学基金(项目号:81525017),题目:流感病原生物学研究~~
【分类号】：R511.7

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