基于核磁共振技术的卵巢静止奶牛血浆代谢谱分析

发布时间：2017-12-26 18:32

本文关键词：基于核磁共振技术的卵巢静止奶牛血浆代谢谱分析　出处：《畜牧兽医学报》2016年01期 　论文类型：期刊论文

【摘要】：为研究卵巢静止奶牛与发情奶牛体内差异代谢物变化,选取产后60~90d的年龄、胎次、体况相近的健康经产高产奶牛作为实验动物。根据奶牛发情表现、直肠检查和B超检查及激素检测的结果,选取10头奶牛为发情组(A),10头奶牛为卵巢静止组(B),然后对两组奶牛血浆样品进行无偏性的1 H谱核磁共振检测,对比其血浆代谢组学图谱,进行多元统计分析,筛选及鉴定卵巢静止奶牛血浆差异代谢产物,并进行生物信息学分析。结果显示:相对于正常发情奶牛,卵巢静止奶牛血浆中共12种代谢产物表现异常,包括水平增加的乙酸、柠檬酸和酪氨酸,以及水平下降的低密度脂蛋白、极低密度脂蛋白、脂质、丙氨酸、丙酮酸、肌酸、胆碱、磷酸胆碱和甘油磷酸胆碱等;这些代谢产物与糖代谢、氨基酸代谢、脂蛋白代谢和胆碱代谢的异常密切相关;通过参与生物合成通路调节,干扰了奶牛正常卵泡生长。并且发现卵巢静止的奶牛血浆肌酸浓度降低和酪氨酸浓度升高的新变化。多种代谢异常提示奶牛卵巢静止与泌乳早期能量负平衡有关,并有伴发酮病和脂肪肝病的风险;另外,卵巢静止奶牛血浆肌酸、胆碱浓度降低和酪氨酸浓度升高的变化,为今后更为深入研究奶牛卵巢静止发生机制提供了新的方向。
[Abstract]:For the study of ovarian quiescence and estrus in dairy cow dairy cows of different metabolites change, selection of postpartum 60~90d age, parity, body condition similar to healthy multiparous cows as experimental animal. According to the findings, oestrus rectal examination and ultrasound examination and hormone test results, select 10 cows for estrus group (A), 10 cows with ovarian quiescence group (B), then the two groups of cows plasma samples were unbiased 1 H NMR detection, the comparison of plasma generation metabolomics map and by multivariate statistical analysis, screening and identification of ovarian static cow plasma metabolite difference, and bioinformatics analysis. The results showed that: compared with normal estrous cows, ovarian static cow plasma metabolites of the 12 abnormalities, including increased levels of acetic acid, citric acid and tyrosine, low density lipoprotein, and decreased levels of very low density lipoprotein, lipid, alanine, pyruvic acid, creatine, choline, phosphocholine and glycerophosphocholine etc.; these metabolites and glucose metabolism, amino acid metabolism, lipid metabolism and choline metabolism is closely related to the abnormal regulation; through participation in the biosynthesis pathway, interferes with the normal follicular growth in dairy cows. And a new change in the decrease of plasma creatine concentration and the increase of tyrosine concentration in the plasma of the cows with the stillness of the ovaries was also found. A variety of metabolic abnormal ovarian quiescence in dairy cows in early lactation and negative energy balance, and is associated with ketosis and fatty liver disease risk; in addition, reduced ovarian static cow plasma creatine, choline concentration change and tyrosine concentration increased, for the future more in-depth study of the mechanism of ovary still provides a new direction.
【作者单位】：黑龙江八一农垦大学动物科技学院;
【基金】：国家自然基金面上项目(31372488) 国家科技支撑计划课题(2013BAD21B01-2;2012BAD12B05;2013BAD21B00) 黑龙江八一农垦大学研究生创新科研项目(YJSCX2015-Y22)
【分类号】：S858.23
【正文快照】： 卵巢静止是奶牛产后乏情的一种常见类型,是卵巢机能不全的一种表现,是由于卵巢机能暂时性扰乱,机能减退,处于静止状态,不出现周期活动。卵巢静止在奶牛卵巢疾病中约占26.3%,在奶牛乏情中发病率最高[1]。其病因主要是长期饲料不足或质量差,特别是蛋白质、维生素A及维生素E的缺

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