NELL1和FMO3基因在绵羊尾脂、皮下脂、肾周脂和肝脏中转录水平表达的研究

发布时间：2018-01-02 18:29

本文关键词：NELL1和FMO3基因在绵羊尾脂、皮下脂、肾周脂和肝脏中转录水平表达的研究　出处：《西北农林科技大学》2016年硕士论文　论文类型：学位论文

更多相关文章：绵羊 NELL1 FMO3 表达 mRNA

【摘要】：绵羊脂肪沉积的部位和沉积方式显著影响羊肉品质和养羊效益。因此,揭示功能基因、日粮及生长阶段对绵羊脂肪沉积的影响,对改良绵羊肉品质具有重要意义。研究选取了脂肪代谢相关功能基因NELL1和FMO3,比较其在不同尾型绵羊品种(哈萨克羊、滩羊、同羊、西藏羊)尾部脂肪组织中的表达差异;探讨营养水平及生长阶段对其在滩羊脂肪组织中表达的影响,旨在揭示不同营养水平及不同生长阶段脂尾型绵羊NELL1和FMO3基因表达的组织特异性及其表达模式,为脂尾型绵羊体内脂肪沉积的营养调控提供理论依据。试验一:不同品种绵羊尾脂中NELL1和FMO3 mRNA表达差异分析选择健康、体重相近的9月龄滩羊、同羊、哈萨克羊和西藏羊羯羊各3只,采集尾部脂肪组织,利用实时荧光定量PCR技术,检测NELL1和FMO3 mRNA表达,结果如下:1.FMO3mRNA在哈萨克羊尾脂中表达量最高(P0.05);在同羊、滩羊、西藏羊尾脂中依次下降,且差异显著(P0.05)。2.NELL1mRNA在西藏羊尾脂中表达量最高(P0.05);在滩羊、同羊、哈萨克羊中表达量依次下降。试验二:营养水平对不同阶段滩羊部分组织中NELL1和FMO3 mRNA表达的影响选择健康4月龄滩羊112只随机分为4组,公母各半,每组四个重复,每个重复7只羊。试验根据增重目标分为3个阶段:第一阶段(22~28kg),第二阶段(28~35kg),第三阶段(35~40kg),每个阶段各组分别饲喂不同营养水平的日粮,由低到高分为T1、T2、T3、T4组。每个生长阶段结束时采集肝脏、肾周脂肪、尾脂、皮下脂肪的组织样品,提取样品总RNA,采用实时荧光定量PCR的方法分析NELL1和FMO3 mRNA的表达差异。结果如下:1.NELL1 mRNA在尾脂中的表达,第1阶段以T4组为最高(P0.05),其他三组差异不显著;第2阶段和第3阶段,也都是以T4组表达量最高(P0.05),T1、T2、T3组表达量略有波动。2.NELL1 mRNA在皮下脂中的表达。第1阶段和第2阶段四个营养水平组间表达差异不显著(P0.05);第3阶段,T4组表达量最高(P0.05),依次为T2、T3、T1组。相同营养水平下,NELL1 mRNA在第1阶段表达量最高(P0.05)。3.NELL1 mRNA在肾周脂中的表达。在同一生长阶段,营养水平对滩羊肾周脂肪组织中NELL1mRNA的表达无显著影响(P0.05);在相同营养水平下,不同生长阶段间表现出表达差异显著(P0.05)。4.NELLL1 mRNA在肝脏中的表达。第1阶段,在四个营养水平组间表达差异不显著;第2阶段,表达以T4组最高(P0.05),依次为T3、T1、T2组;第3阶段,表达以T1组为最高(P0.05),依次为T2、T3、T4组。3个阶段表达依次升高,其中第3阶段表达量最高(P0.05)。5.FMO3 mRNA的表达。尾部脂肪组织中最高表达均出现在T2组;在第1阶段、第2阶段和第3阶段,T1、T2、T3、T4组中表达量均呈现先升高再降低的趋势;皮下脂肪组织T3组最高,肾周脂最高表达出现在T1组,肝脏的最高表达出现在第T4组,最高表达组与相同阶段时的最低表达组相比差异显著的(P0.05)。试验三:NELL1、FMO3mRNA在滩羊不同组织中的表达差异分析在试验第三阶段结束当天,选取T2组4只滩羊进行屠宰,采集肝脏、肾周脂肪、尾脂、皮下脂肪组织,利用实时荧光定量PCR技术,检测NELL1、FMO3mRNA的相对表达量,结果如下:1.NELL1 mRNA在滩羊尾部脂肪组织、皮下脂肪组织、肾周脂肪组织、肝脏中均有表达。NELL1在肝脏组织中表达最高,显著高于尾脂、皮下脂和肾周脂(P0.05),其次是在肾周脂中的表达,高于尾脂和皮下脂(P0.05),在尾脂和皮下脂中的表达最低,两者之间差异不显(P0.05)。2.FMO3 mRNA在滩羊尾部脂肪组织、皮下脂肪组织、肾周脂肪组织、肝脏中均有表达。FMO3在肝脏组织中表达最高,显著高于尾部脂肪组织、皮下脂肪组织和肾周脂肪组织(P0.05),其次是在尾部脂肪组织中的表达,高于肾周脂肪组织和皮下脂肪组织(P0.05),在肾周脂肪组织和皮下脂肪组织中的表达最低,两者之间差异不显(P0.05)。综合以上试验结果,表明NELL1和FMO3基因在绵羊组织中的表达受到品种、部位、生长阶段、营养水平等原因会有不同的表达结果,其表达差异是与脂肪的沉积差异是存在一定关系。
[Abstract]:Location and mode of deposition of sheep fat deposition significantly affect the quality of mutton and sheep benefits. Therefore, to reveal the functional gene, the effects of dietary and growth stages of sheep fat deposition, and has the important significance for improving the quality of sheep meat. The fat metabolism related genes NELL1 and FMO3, the tail type in different sheep breeds (Kazak sheep, Tan sheep, Tong sheep, Tibet sheep) differentially expressed in adipose tissue of the tail; nutrition level and growth effects on its expression in sheep adipose tissue, to reveal the different nutritional levels and different growth stages of fat tail sheep NELL1 and expression of FMO3 gene and tissue specific expression pattern. To provide a theoretical basis for the nutritional regulation of fat deposition of fat tail sheep. Experiment one: compare health NELL1 and the expression of mRNA FMO3 in different varieties of sheep tail fat, body weight were 9 Month old Tan sheep, Tong sheep, Kazak sheep and Tibet sheep were all 3, collected tail fat tissue, using real-time fluorescence quantitative PCR technology, detection of NELL1 and FMO3 mRNA expression, the results are as follows: 1.FMO3mRNA in Kazak sheep tail fat expression was the highest (P0.05); in the same Tan sheep, Tibet sheep tail fat. In order to decrease, and the difference was significant (P0.05).2.NELL1mRNA in Tibet sheep tail fat the highest expression level (P0.05); in the Tan sheep, Tong sheep, Kazakstan sheep in the amount of expression gradually decreased. Experiment two: the influence of nutrition level on the expression of NELL1 and FMO3 mRNA part of the organization in different stages of selection in the April health sheep old Tan sheep were randomly 112 divided into 4 groups, male and female, with four replicates, each replicate 7 sheep. The test is divided into 3 stages according to the weight target: the first stage (22~28kg), the second stage (28~35kg), the third stage (35~40kg), each stage of the four groups were fed with different nutritional levels of the day Grain, from low to high as T1, T2, T3, T4 group. The end of each growth stage when collecting the liver, perirenal fat, fat tail, subcutaneous adipose tissue samples were extracted total RNA expression by real-time quantitative PCR analysis of NELL1 and FMO3 mRNA. The results are as follows: the expression of 1.NELL1 mRNA in the first stage of fat tail, T4 group was the highest (P0.05), the other three groups had no significant difference; the second and third stages, is also the highest expression level in T4 group (P0.05), T1, T2, T3 group, the expression of.2.NELL1 mRNA in slight fluctuations in subcutaneous fat in first stages. And the second stage four nutrition level group. The expression difference was not significant (P0.05); the third stage, the highest expression level in T4 group (P0.05), followed by T2, T3, T1 group. The same nutrient level, NELL1 mRNA expression was the highest in the first stage (P0.05) expression of.3.NELL1 mRNA in kidney at the same Zhou Zhizhong. A period of growth, nutrition level of Tan sheep No significant effect on the expression of NELL1mRNA in perirenal adipose tissue (P0.05); in the same nutrient level, different growth stages showed significant differences in the expression of.4.NELLL1 mRNA (P0.05) expression in the liver. In the first stage, in the four trophic level group. The expression difference was not significant; the second stage, the expression of T4 was the highest (P0.05), followed by T3, T1, T2 group; the third stage, the expression in T1 group was the highest (P0.05), followed by T2, T3, T4 group.3 expression gradually increased, the third stage of the highest expression level (P0.05). The expression of.5.FMO3 mRNA were found in T2 group is the highest expression of fat tail fat tissue; in the first stage, second stage and third stage, T1, T2, T3, T4 expression in the group volume showed first increased and then decreased; subcutaneous adipose tissue of T3 group was the highest, perirenal fat was the highest in T1 group, the highest expression in the liver of T4 group, the highest expression group with the same stage of the lowest The expression was significantly different (P0.05). Experiment three: NELL1 analysis of expression of FMO3mRNA in different tissues of sheep in the third trial of the end of the day, T2 group selected 4 Tan sheep were slaughtered, collected liver, perirenal fat, fat tail, subcutaneous adipose tissue, detection of NELL1 using real-time quantitative PCR technique and the relative expression of FMO3mRNA, the results are as follows: 1.NELL1 mRNA in sheep tail fat, subcutaneous adipose tissue, perirenal adipose tissue, the expression of.NELL1 had the highest expression in the liver tissue in the liver, was significantly higher than that of tail fat, subcutaneous fat and perirenal fat (P0.05), followed by the expression of perirenal Zhizhong, higher than tail fat and subcutaneous fat (P0.05), the lowest expression in tail fat and subcutaneous fat in between the two, the difference was not significant (P0.05).2.FMO3 mRNA in sheep tail fat, subcutaneous adipose tissue, perirenal adipose tissue,.FMO3 was expressed in the liver in the liver The highest expression was significantly higher than that of the tail, adipose tissue, subcutaneous adipose tissue and perirenal adipose tissue (P0.05), the second is expressed in tail fat tissue, higher in perirenal adipose tissue and subcutaneous adipose tissue (P0.05), in perirenal adipose tissue and subcutaneous adipose tissue was the lowest, the difference between the two no significant (P0.05). These test results showed that the expression of NELL1 and FMO3 gene in sheep tissues by species, site, growth stage, nutrient levels and other reasons will result expression of different, its expression difference is and there are differences in fat deposition.

【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S826

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