辽宁部分鹅场沙门氏菌的分离鉴定及耐药性的分析

发布时间：2018-01-03 15:04

本文关键词：辽宁部分鹅场沙门氏菌的分离鉴定及耐药性的分析　出处：《沈阳农业大学》2016年硕士论文　论文类型：学位论文

更多相关文章： 沙门氏菌 InvA 血清型 耐药性 鹅

【摘要】：随着辽宁地区养鹅业的飞速发展及家禽沙门氏菌病的多发,众多养鹅场和种鹅育种基地对鹅沙门氏菌病的防治工作越发重视,但目前鲜见对辽宁省内鹅场的沙门氏菌病发生发展情况进行调查的研究报告。本研究挑选辽宁部分地区6家鹅场,每家鹅场随机选取20-30只种鹅,采集164只鹅血液及泄殖腔棉拭子样本。现场对抽检鹅进行沙门氏菌多价染色平板抗原快速检测；将采集的泄殖腔棉拭子带回实验室分别进行选择培养基分离鉴定、生化鉴定、PCR及血清型检测,并对获得的鹅源沙门氏菌分离株进行耐药性检测分析。本研究结果如下：1.现场采集鹅全血进行沙门氏菌多价染色平板抗原快速检测发现,164只抽检鹅有118只鹅携带沙门氏菌抗体,检出率为72%。2.采用沙门氏菌选择培养基从抽检的164份鹅泄殖腔棉拭子中筛选出63株符合沙门氏菌鉴别培养基特性的菌株,再对疑似菌株进行沙门氏菌生化鉴定,筛出26株符合沙门氏菌生化特性的菌株。3.对26株符合沙门氏菌生化特性的菌株分别进行血清型和PCR检测,共检出12株均带有InvA基因的沙门氏菌菌株,分别为甲型副伤寒沙门氏菌(3株)、乙型副伤寒沙门氏菌(1株)、丙型副伤寒沙门氏菌(3株)、汤卜逊沙门氏菌(1株)、德比沙门氏菌(1株)、阿贡那沙门氏菌(2株)、伤寒沙门氏菌(1株)；其中1株带有沙门氏菌肠毒素STN基因。4.对26株符合沙门氏菌生化特性的菌株进行耐药性检测,发现大部分菌株对青霉素、阿莫西林、林可霉素、四环素、甲硝唑、复方新诺明有着较强的耐药性；对头孢哌酮、庆大霉素、卡那霉素、多粘菌素B有着较强的敏感性。本研究表明,辽宁地区大部分鹅场已出现沙门氏菌的感染现象,并有较多耐药菌株出现,提示生产实践中一定要建立合理的饲养和管理制度,减少抗生素的使用,并注意监测种鹅沙门氏菌的带菌情况。试验结果可为临床防治鹅沙门氏菌病提供依据。
[Abstract]:With the rapid development of goose breeding in Liaoning and the frequent occurrence of salmonella in poultry, more and more attention has been paid to the control of goose salmonellosis in many goose farms and breeding bases. However, there are few reports on the investigation of the occurrence and development of salmonellosis in geese farms in Liaoning Province. In this study, 6 geese farms in some areas of Liaoning Province were selected and 20-30 geese were randomly selected from each goose farm. Blood samples and cloacal cotton swabs were collected from 164 geese. The collected cloacal cotton swabs were taken back to the laboratory for isolation and identification of selective culture medium, biochemical identification, PCR and serotype detection. The results of this study are as follows: 1. The whole blood of goose was collected to detect the polyvalent plate antigen of salmonella. 118 of 164 geese carried salmonella antibodies. The detection rate was 72.63 strains of salmonella were selected from 164 goose cloacal cotton swabs by using salmonella selective medium. 26 strains of Salmonella were identified by biochemical identification of Salmonella and 26 strains with biochemical characteristics of Salmonella were tested for serotype and PCR. A total of 12 Salmonella strains with InvA gene were identified, including 3 strains of Salmonella paratyphi A, 1 strain of Salmonella paratyphi B and 3 strains of Salmonella paratyphi C. One strain of Salmonella Thomson, one strain of Derby Salmonella, two strains of Argona Salmonella, one strain of Salmonella typhimurium; One of them had the STN gene of Salmonella enterotoxin. 26 strains that accord with the biochemical characteristics of Salmonella were tested for drug resistance and most of them were found to be penicillin, amoxicillin and lincomycin. Tetracycline, metronidazole and compound sulfamide have strong drug resistance. Cefoperazone, gentamycin, kanamycin, polymyxin B have a strong sensitivity. This study showed that most goose farms in Liaoning Province have the phenomenon of Salmonella infection, and there are more drug-resistant strains. It is suggested that a reasonable feeding and management system should be established in production practice, the use of antibiotics should be reduced, and attention should be paid to the monitoring of the carrying status of Salmonella geese. The results of the experiment can provide a basis for clinical prevention and treatment of goose salmonella.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S852.61

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