紫锥菊多糖对LPS损伤后IEC-6分泌IL-6mRNA的影响（英文）

发布时间：2018-01-16 07:15

  本文关键词：紫锥菊多糖对LPS损伤后IEC-6分泌IL-6mRNA的影响（英文）　出处：《Agricultural Science & Technology》2016年11期 　论文类型：期刊论文

  更多相关文章： 紫雏菊多糖 肠道疾病 免疫 调节

【摘要】：[目的]探讨紫锥菊多糖(EPS)在内毒素(LPS)损伤后IEC-6分泌IL-6m RNA的影响。[方法]采用Trizon试剂提取总RNA,经RT-PCR扩增后,进行琼脂糖凝胶电泳及图像分析。[结果]当LPS刺激IEC-6细胞时,IL-6m RNA分泌增加。而EPS可以抑制这种作用,并且具有剂量依赖性,50μg/ml EPS可以部分抑制LPS刺激IEC-6产生的IL-6水平,而100、500μg/ml EPS随着浓度的增加,其抑制IL-6的水平逐渐增加;将IEC-6用50、100、200、500μg/ml EPS预处理24 h,然后用LPS(10μg/ml)刺激1、4h,采用RT-PCR方法分析得,LPS诱导IL-6m RNA表达被EPS抑制且具有时间依赖性。[结论]证实了LPS作用于小肠上皮细胞后,其分泌的细胞因子IL-6m RNA产生增多,而EPS可以抑制LPS刺激细胞分泌的IL-6m RNA的产生从而起到肠道粘膜的保护作用,并且这种抑制作用具有浓度及时间依赖性。
[Abstract]:[Objective] to investigate the Echinacea polysaccharide (EPS), (LPS)] IL-6m RNA. Methods IEC-6 secretion after injury to extract total RNA using Trizon reagent, amplified by RT-PCR, agarose gel electrophoresis and image analysis. Results when LPS stimulated IEC-6, IL-6m and EPS increased secretion of RNA. Can inhibit this effect, and in a dose dependent manner. 50 g/ml EPS could inhibit LPS stimulated IEC-6 production level of IL-6, and 100500 g/ml EPS with the increase of concentration, the inhibition of IL-6 levels gradually increased; the IEC-6 with 50100200500 g/ml EPS pretreatment for 24 h, then LPS (10 g/ml) stimulated 1,4h analysis by using RT-PCR method, LPS IL-6m induced RNA expression was inhibited by EPS and a time dependent manner. Conclusion] confirmed the effect of LPS on intestinal epithelial cells, the secretion of cytokines of IL-6m increased RNA production, and EPS can inhibit LPS cell stimulation The production of the secreted IL-6m RNA plays a protective role in the intestinal mucosa, and this inhibitory effect is dependent on concentration and time.

【作者单位】： 河北省预防兽医学重点实验室/河北科技师范学院;永清县畜牧兽医局;
【基金】：Supported by National Natural Science Foundation of China(31472230) Natural Science Foundation of Hebei Province(C2014407068) Project of Science and Technology Department of Hebei Province(14966610D)~~
【分类号】：S853.7
【正文快照】： Traditional Chinese herb Echi-ing in bacterial translocation and in-nacea purpurea has extensivetestinal endotoxemia,thereby furtherpractice in the prevention andexacerbating internal epithelial celtreatment of infectious diseases indamage.Under that con，

本文编号：1432070