火鸡多杀性巴氏杆菌的分离鉴定及致病性研究

发布时间：2018-01-16 09:07

本文关键词：火鸡多杀性巴氏杆菌的分离鉴定及致病性研究　出处：《中国兽医科学》2016年02期 　论文类型：期刊论文

【摘要】：2014年8月长春某观赏动物园区的火鸡发生急性死亡,临床诊断疑似为多杀性巴氏杆菌感染。分离的病原经染色鉴定为革兰阴性、美兰染色呈两极浓染的球杆菌,Kmt基因序列分析发现该病原与多杀性巴氏杆菌的同源性为99%,PCR分型鉴定为荚膜A型巴氏杆菌。致病性结果表明,该分离菌对鸡表现出较高的致病性,人工感染4周龄雏鸡的LD_(50)为16 CFU。药敏试验结果显示,该菌对阿米卡星、米诺环素、头孢哌酮、庆大霉素敏感,经敏感抗生素紧急注射结合严格消毒措施控制了该病的发生和蔓延。
[Abstract]:In August 2014, an acute death occurred in a turkey in an ornamental zoo in Changchun, which was clinically diagnosed as Pasteurella multocida infection. The isolated pathogen was identified as Gram-negative by staining. The sequence analysis of the Kmt gene of Streptococcus spp in methylene blue staining showed that the homology between the pathogen and Pasteurella multocida was 99m PCR and identified as Pasteurella perfringens A. The pathogenicity of the pathogen showed that the pathogen was homologous to Pasteurella multocida. The isolated strain showed high pathogenicity to chicken, and LDX 50 of 4-week-old chicks infected artificially was 16 CFU. The drug sensitivity test showed that the strain was resistant to amikacin, minocycline and cefoperazone. The occurrence and spread of gentamicin were controlled by emergency injection of sensitive antibiotics and strict disinfection measures.
【作者单位】：吉林农业大学动物科学技术学院;长春动植物公园;吉林农业大学中药材学院;
【基金】：吉林农业大学博士启动基金项目
【分类号】：S858.39
【正文快照】： 禽霍乱是由多杀性巴氏杆菌引起禽类的一种常发性疾病,可以感染所有禽类且多为致死性感染[1]。16周龄以上禽类易感性更高,成年火鸡最为易感,发病最为严重。该病广泛流行于世界各地,健康携带病原或感染的禽类均是重要的传染源[2-3]。急性型禽霍乱可导致动物败血症、肝损伤和肠道

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