钼镉联合诱导对麻鸭肝脏线粒体抗氧化功能及部分凋亡基因的影响

发布时间：2018-01-19 11:39

本文关键词： 鸭钼镉肝脏凋亡基因　出处：《江西农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本试验通过研究钼镉联合诱导对麻鸭肝脏线粒体抗氧化功能、肝脏超微结构及部分凋亡基因表达的影响,来探讨钼、镉之间的毒理学关系。本试验以钼酸铵[(NH4)6Mo7O24·4H2O]作为钼源、以硫酸镉(3CdSO4·8H2O)作为镉源;将360羽11日龄“江南2号”麻鸭随机分成6组(每组公母各30羽):各组在每千克基础日粮中添加Mo、Cd的剂量分别为:I组:对照组(Mo 0mg,Cd 0mg)、II组:低钼组(Mo 15mg,Cd 0mg)、III组:高钼组(Mo 100mg,Cd 0mg)、IV组:镉组(Mo 0mg,Cd 4mg)、V组:低钼镉组(Mo 15mg,Cd 4mg)、VI组:高钼镉组(Mo 100mg,Cd 4mg)。试验周期为120d,分别在试验的第30d、60d、90d、120d每组随机选10羽鸭(公母各5羽)进行剖杀并采集所需样品;检测肝脏线粒体MDA含量及CAT、NOS、SOD、XOD、T-AOC活性;检测血清中TG、HDL-c、LDL-c、DBIL、IBIL、CP的含量;检测肝脏凋亡基因Bcl-2、Bak-1和Caspase-3 mRNA的表达量及肝脏细胞超微结构变化。试验结果如下:1.除低钼组外,肝脏线粒体的XOD、CAT活性在整个试验均显著低于对照组(P0.05);T-AOC、SOD活性在60d、90d、120d显著低于对照组(P0.05);MDA、NOS含量显著高于对照组(P0.05);且钼镉联合组与单独组比较差异显著(P0.05)。2.除低钼组外,血清TG、LDL-c、DBIL、IBIL的含量较对照组呈显著上升的趋势(P0.05);HDL-c、CP较对照组呈显著下降趋势(P0.05);且钼镉联合组与单独组比较差异显著(P0.05)。3.除低钼组外,肝脏Bak-1、Caspase-3基因表达量较对照组呈显著上升趋势(P0.05);试验组Bcl-2基因表达量较对照组呈显著下降趋势(P0.05),且钼镉联合组与单独组比较差异显著(P0.05)。4.第120d的镉组、高钼组和高钼镉组的肝脏细胞超微结构出现了核固缩、线粒体肿胀、空泡化以及嵴断裂等病理变化。结论:钼、镉及其联合诱导均会导致麻鸭肝脏线粒体发生抗氧化功能损伤;并且导致肝脏超微结构出现核固缩、线粒体肿胀、空泡化以及嵴断裂等病理变化;还导致了肝脏中Bak-1mRNA、capase-3mRNA表达上升和Bcl-2 mRNA表达下降,并且整体钼镉呈协同作用。
[Abstract]:In this study, the toxicological relationship between molybdenum and cadmium was studied by studying the effects of molybdenum and cadmium combined induction on antioxidant function, liver ultrastructure and partial apoptotic gene expression in liver mitochondria of Ma duck. In this experiment, ammonium molybdate was used to study the toxicological relationship between molybdenum and cadmium. [NH _ 4O _ 6Mo _ 7O _ 24 路4H _ 2O] was used as molybdenum source, and cadmium sulfate was used as CD _ 2O _ 3CdSO _ 4 路8H _ 2O source. 360 11-day-old "Jiangnan No.2" ducks were randomly divided into 6 groups (30 male and female in each group): Mo was added to the basal diet per kilogram in each group. The dosages of CD were as follows: group I: control group: control group: Mo 0 mg / g CD 0 mg / g; group II: low molybdenum group: Mo 15 mg / g CD 0 mg). III group: high molybdenum group, Mo 100 mg / CD, CD 0 mg, group IV, cadmium group, Mo 0 mg, CD 4 mg / L, group V, low molybdenum and cadmium group, Mo 15 mg. CD 4 mg / L group VI: high molybdenum and cadmium group, Mo 100 mg / L CD 4 mg / L. The test period was 120 days, and the test period was 90 days on the 30th day of the experiment. 10 ducks (5 male and 5 female) were randomly selected for 120 days and the samples were collected. The content of MDA in liver mitochondria and the activity of XODT-AOC were measured. To detect the content of BILCP in serum of TGN HDL-cr. The expression of apoptotic genes Bcl-2, Caspase-3 mRNA and ultrastructure of liver cells were detected. The results were as follows: 1. Except for low molybdenum group. The activity of XODcat in liver mitochondria was significantly lower than that in control group (P 0.05). The activity of SOD in T-AOC was significantly lower than that in the control group (P 0.05) at 60d ~ 90d ~ 120d. The content of nitric oxide synthase (NOS) in MDAN was significantly higher than that in control group (P 0.05). In addition to the low molybdenum group, the serum TGN LDL-cn DBIL was significantly different between the molybdenum and cadmium combined group and the single group. Compared with the control group, the content of IBIL increased significantly (P 0.05). Compared with the control group, HDL-cn CP decreased significantly (P 0.05). The difference between the molybdenum and cadmium combined group and the single group was significant (P 0.05). In addition to the low molybdenum group, liver Bak-1 was significantly different. Compared with the control group, the expression of Caspase-3 gene increased significantly (P 0.05). Compared with the control group, the expression of Bcl-2 gene in the trial group was significantly decreased, and the difference between the molybdenum and cadmium combination group and the single group was significant (P0.05. 4). The cadmium group at the 120th day showed significant difference. The ultrastructure of liver cells in high molybdenum group and high molybdenum cadmium group showed nuclear shrinkage, mitochondria swelling, vacuolation and cristal rupture. Cadmium and its combined induction could lead to the damage of antioxidant function in liver mitochondria of duck. The pathological changes of liver ultrastructure such as pyknosis, swelling of mitochondria, vacuolation and cristal fracture were also found. The expression of Bak-1 mRNA-capase-3 mRNA and the expression of Bcl-2 mRNA in liver were increased, and the whole molybdenum and cadmium showed synergistic effect.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S859.8

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