寿光鸡AANAT基因5′调控区的单核苷酸多态性及其与产蛋性状的关联分析

发布时间：2018-01-20 05:10

本文关键词： 寿光鸡 -羟色胺-N-乙酰基转移酶基因(AANAT) 产蛋性状 SNPs 关联分析　出处：《农业生物技术学报》2017年06期 　论文类型：期刊论文

【摘要】：褪黑激素(melatonin,Mel)分泌增加可阻碍家禽生殖器官的发育,降低家禽产蛋量。5-羟色胺-N-乙酰基转移酶(arylalkylamine N-acetyltransferase,AANAT)是Mel合成过程中的限速酶。本实验旨在研究AANAT基因5'调控区多态性及其与寿光鸡(Gallus domesticus'Shouguang')产蛋性能的关系,采用直接测序法检测了AANAT基因5'调控区的SNPs,并将检测到的SNPs与寿光鸡的产蛋性状进行关联分析。结果表明,AANAT基因5'控区共21个SNPs,其中10个低度多态、11个中度多态。3个中度多态位点对产蛋性能影响显著(P0.05),其中-803位点能使开产日龄显著提前,并能使前期(20~25周龄)产蛋数显著增加;-561位点能使中期产蛋数显著增加;-522位点能使后期(46~57周龄)产蛋数显著增加;-561、-522位点能使产蛋总数显著增加。综上所述,AANAT基因5'调控区有多个SNPs对产蛋性状影响显著,可作为寿光鸡产蛋性状辅助选择的有潜在利用价值的分子遗传标记。
[Abstract]:The increased secretion of melatonin (melatonin) may hinder the development of reproductive organs in poultry. Decreased egg production. 5-hydroxytryptamine N-acetyltransferase (Arylalkylamine N-acetyltransferase). Aanat is a rate-limiting enzyme in the process of Mel synthesis. The aim of this study was to study the polymorphism of 5 'regulatory region of AANAT gene and its association with Shouguang chicken (. The relationship between Gallus domesticus and egg laying performance. SNPs of 5 'regulatory region of AANAT gene were detected by direct sequencing, and the results showed that the detected SNPs was associated with egg laying traits of Shouguang chicken. There were 21 SNPs in the 5'control region of AANAT gene, of which 10 were low polymorphic, 11 were moderate polymorphic, and 3 moderate polymorphic loci had significant effect on egg laying performance (P0.05). The -803 locus could significantly advance the first day and increase the number of eggs at 20 ~ 25 weeks. -561 locus could significantly increase the number of eggs laid in the middle stage. -522 locus could significantly increase the number of eggs laid at the later age of 46 ~ 57 weeks. The results showed that the total number of eggs was significantly increased at -561 ~ 522 locus. In conclusion, there were more than one SNPs in the 5 '-regulatory region of the AANAT gene, which had a significant effect on the egg laying traits. It can be used as a potentially useful molecular genetic marker for assisted selection of egg-laying traits in Shouguang Chicken.
【作者单位】：山东农业大学动物科技学院;
【基金】：山东省现代农业产业技术体系项目(No.SDAIT-11-02)
【分类号】：S831.2
【正文快照】： 褪黑激素(melatonin,Mel)主要是由松果体和视网膜产生的一种吲哚类化合物(雷梦媛等,2015;赵雪丹等,2014),在哺乳动物及鸟类的多种组织广泛分布,是光照信息同生殖系统协调关系中的重要信号(辛海瑞等,2016),可抑制下丘脑-垂体-性腺轴,使促性腺激素和促卵泡激素等的分泌减少、促

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