茶皂素对PRRSV感染Marc-145细胞受体表达及Caspase-9活化的影响

发布时间：2018-01-20 07:58

本文关键词： PRRSV 茶皂素病毒穿入 CD163 Vimentin Caspase-9　出处：《山西农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的：以茶皂素(Tea saponin, TS)作为候选药物,通过研究茶皂素对Marc-145细胞受体CD163和波形蛋白(Vimentin)基因合成和蛋白表达是否有影响,以及茶皂素是否能通过细胞凋亡内源性通路影响PRRSV感染细胞,初步探究茶皂素的抗病毒作用机制。方法：通过qRT-PCR技术研究TS对细胞受体CD163和Vimentin基因合成的影响。同时运用Western blot技术检测TS对细胞受体CD163和Vimentin蛋白表达量的影响。另外,通过Western blot技术研究TS对细胞内源性凋亡通路启动子Caspase-9活化的影响,初探TS的抗病毒机制。结果：1.qRT-PCR结果表明TS能显著抑制感染PRRSV的Marc-145细胞受体CD163和Vimentin基因的合成。2.Western blot结果表明TS能显著抑制细胞受体CD163和Vimentin的蛋白表达。3.Western blot结果表明TS能够引起细胞内源性凋亡通路启动子Caspase-9的活化。结论：1.TS能抑制PRRSV在Marc-145细胞上的穿入过程,从而达到抗PRRSV的作用。2.TS可能通过激活细胞凋亡内源性通路以早期促进细胞凋亡的方式,而产生抗PRRSV的作用。
[Abstract]:Objective: to use tea saponin (TSX) as a drug candidate. The effects of tea saponin on gene synthesis and protein expression of Marc-145 receptor CD163 and vimentin were studied. And whether tea saponin can affect PRRSV infected cells through the endogenous pathway of apoptosis. To explore the antiviral mechanism of tea saponin. Methods:. The effects of TS on the synthesis of CD163 and Vimentin genes were studied by qRT-PCR. Meanwhile, Western was used to study the effect of TS on the synthesis of CD163 and Vimentin genes. Blot technique was used to detect the effect of TS on the expression of CD163 and Vimentin protein. The effects of TS on the activation of Caspase-9 promoter of intracellular apoptotic pathway were studied by Western blot technique. Preliminary study on antiviral mechanism of TS. Results:. 1. The results of qRT-PCR showed that TS could significantly inhibit the synthesis of CD163 and Vimentin genes of Marc-145 cells infected with PRRSV. The results of blot showed that TS could significantly inhibit the protein expression of CD163 and Vimentin. 3. The results of blot showed that TS could induce the activation of Caspase-9 in the endogenous apoptotic pathway. 1. TS could inhibit the penetration of PRRSV in Marc-145 cells. TS may promote apoptosis by activating endogenous pathway of apoptosis, which may have the effect of anti-#en1#. 2.
【学位授予单位】：山西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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