深县猪保种群遗传多样性简化监测方法研究

发布时间：2018-01-20 21:27

本文关键词： 深县猪遗传多样性监测方法指标体系　出处：《中国畜牧兽医》2017年10期 　论文类型：期刊论文

【摘要】：为建立深县猪种群多样性监测的简化指标体系,试验采集河北省深县猪4个世代179头猪样本,采用联合国粮农组织和国际动物遗传协会联合推荐的27个微卫星标记进行群体遗传多样性分析,对27个微卫星标记进行两阶段、多梯度抽样,并根据抽样群体和总群体的多态信息含量(PIC)值的变化确定适宜抽样微卫星位点数,再以4个世代样本进行验证。结果表明,选取8个微卫星位点时与全部位点的比较相对偏差可控制在5%以内,且以各分世代的验证比较相对偏差在10%左右,通过比较确定以S0155、S0228、SW632、S0090、CGA、S0101、S0227、SW122这8个微卫星位点构成的简化指标体系可较好地反映深县猪种群遗传多样性的变化。因此,深县猪的保种群群体遗传多样性监测可以简化为检测这8个微卫星位点,同时本研究采用的方法也可为其他保种群的遗传多样性监测方法的简化处理提供参考。
[Abstract]:In order to establish a simplified index system for monitoring the population diversity of pigs in Shenxian County, 179 pig samples from 4 generations of pigs in Shenxian County, Hebei Province were collected. The 27 microsatellite markers recommended jointly by FAO and the International Association of Animal Genetics were used for population genetic diversity analysis, and 27 microsatellite markers were sampled in two stages with multi-gradient sampling. According to the variation of the polymorphic information content (PICs) of the sampling population and the total population, the suitable number of microsatellite sites for sampling was determined, and the results were verified by four generations of samples. The relative deviation between 8 microsatellite loci and all the loci can be controlled within 5%, and the relative deviation of comparison between each generation is about 10%, and S0155 is determined by comparison. S0228,SW632,S0090,CGA,S0101,S0227. The simplified index system composed of 8 microsatellite loci of SW122 can well reflect the variation of genetic diversity of pig population in Shenxian county. The genetic diversity monitoring of preserved populations in Shenxian pig population can be simplified to detect the 8 microsatellite loci, and the method used in this study can also provide a reference for the simplification of genetic diversity monitoring methods of other protected populations.
【作者单位】：河北农业大学动物科技学院;国家北方山区农业工程技术研究中心;河北省山区农业工程技术研究中心;辛集市正农牧业有限公司;
【基金】：河北省现代农业产业技术体系专项(HBCT2013070202) 河北省大学生创新创业训练计划项目(201610086028) 河北农业大学大学生创新创业训练计划项目(20160401)
【分类号】：S828
【正文快照】： 群体遗传多样性是家畜遗传资源的重要内容,反映了群体内部不同个体的遗传变异总和的大小及该群体进化的趋势。保种群遗传多样性的稳定性反映了保种工作的进展和质量,只有保种群的遗传多样性相对稳定,才能确定保种成功。开展保种群体的连续性遗传多样性监测,能够及时且动态的了

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