猪伪狂犬病病毒抗体间接ELISA检测方法的建立

发布时间：2018-01-21 00:43

本文关键词： 伪狂犬病毒 gE 间接ELISA　出处：《中国预防兽医学报》2017年06期 　论文类型：期刊论文

【摘要】：为建立一种快速检测猪伪狂犬病病毒(PRV)的血清学方法,本实验采用原核重组表达的g E蛋白作为包被抗原,并对各反应条件进行优化,建立了检测PRV血清抗体的间接ELISA方法。以该方法检测PRV阳性血清和其它猪病病原阳性血清,除了PRV阳性血清呈阳性外,对其它猪病病原阳性血清的检测结果均为阴性,表明该方法具有良好的特异性。批内和批间重复试验的最大变异系数分别是5.95%和9.62%。此外,使用该方法对250份临床血清进行检测,与国内外4种商品化试剂盒的检测结果比较符合率最高为83.6%。表明该方法可以用于PRV的临床检测,为PRV流行病学调查和防控提供了一种快速有效的检测方法。
[Abstract]:In order to establish a rapid serological method for the detection of porcine pseudorabies virus (PRV), the recombinant prokaryotic expression of g E protein was used as the coated antigen, and the reaction conditions were optimized. An indirect ELISA method for the detection of PRV serum antibodies was established. The PRV positive serum and other pathogenic sera of swine diseases were detected by this method, except for the PRV positive serum. The positive sera of other swine diseases were all negative, which indicated that the method had good specificity. The maximum coefficients of variation of repeated tests were 5.95% and 9.62, respectively. This method was used to detect 250 clinical serum samples, the highest coincidence rate was 83.6 compared with the results of four commercial kits at home and abroad. The results showed that this method could be used for clinical detection of PRV. It provides a rapid and effective method for PRV epidemiological investigation and prevention and control.
【作者单位】：华南农业大学兽医学院;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;
【基金】：国家重点研发计划项目(2016YFD0500700) 广东省科技计划项目(2015B020230009)
【分类号】：S858.28
【正文快照】： 猪伪狂犬病毒(Pseudorabies viru,PRV)为疱疹病毒科α-疱疹病毒亚科双链DNA病毒。最早在1813年发现于美国。该病首次发生于牛,被称为“疯痒病”,特点是重度瘙痒[1-2]。能够引起多种动物发生以发热、奇痒、脑脊髓炎为主要特征的传染病,PRV宿主谱非常广泛,可以感染除人和高等灵

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