METTL3对乳腺上皮细胞中乳蛋白和乳脂肪合成的影响

发布时间：2018-01-21 08:51

本文关键词： m A甲基转移酶奶牛乳腺上皮细胞乳脂肪乳蛋白　出处：《中国畜牧兽医》2017年05期 　论文类型：期刊论文

【摘要】：本试验旨在研究m6 A甲基转移酶(methyltransferase-like protein 3,METTL3)在奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMEC)中对乳蛋白和乳脂肪的调节作用。试验建立不同的BMEC模型,应用Western blotting、免疫荧光法检测添加雌激素(E)、蛋氨酸(Met)、METTL3超表达和抑制时对BMEC中乳蛋白和乳脂的影响,以及基因定位和表达变化。结果显示,当在BMEC培养液中添加E、Met及METTL3表达水平提高时,mTOR、p-mTOR、GlyRS、p-GlyRS、CSN2、SREBP-1c等表达量均增加,反之,这些基因表达量均下降,表明METTL3可以通过调控乳脂、乳蛋白的相关信号通路分子的基因表达进而影响乳脂、乳蛋白的表达。提示METTL3是乳合成的重要调节分子,可对细胞中乳脂、乳蛋白的合成起正调控作用。
[Abstract]:The aim of this study was to study m6A methyltransferase-like protein 3. METTL3) in bovine mammary epithelial cells. Different BMEC models were established, Western blotting and immunofluorescence were used to detect the addition of estradiol (E). The effects of METTL3 on the expression of lactoprotein and milk fat in BMEC, as well as the changes of gene location and expression during the inhibition of METTL3. The results showed that when E was added to the BMEC medium, the effect of METTL3 on the gene location and expression was observed. When the expression level of Met and METTL3 increased, the expression of mTORP p-mTORG was increased, whereas the expression of SREBP-1c was increased. These genes were all decreased, indicating that METTL3 can affect the expression of milk fat by regulating the gene expression of the signaling pathway molecules related to milk fat and milk protein. The expression of milk protein suggests that METTL3 is an important regulating molecule of milk synthesis and can play a positive role in the synthesis of milk fat and milk protein.
【作者单位】：东北农业大学乳品科学教育部重点实验室;
【基金】：国家自然科学基金项目(31472162) 国家高技术研究发展计划(863计划)项目(2013AA102504-03)
【分类号】：S823
【正文快照】： 近年来DNA甲基化作为一种重要的表观遗传标记,在基因功能调控中的作用得到了广泛认可。而RNA甲基化属于转录后水平调控,RNA研究成为科学界快速兴起的热门领域,尤其是N6-甲基腺嘌呤(N6-methyladenosine,m6 A)[1-2]。m6 A是mR-NA上含量最高的中间化学修饰,对RNA加工代谢具有重要

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