无菌动物在奶牛乳房炎发病机理研究中的应用

发布时间：2018-01-22 01:28

本文关键词： 奶牛肠道菌群乳房炎无菌小鼠菌群移植　出处：《中国实验动物学报》2017年06期 　论文类型：期刊论文

【摘要】：乳房炎是奶牛养殖场中的高发病,经抗生素治愈后极易复发。疾病的反复发作导致奶牛的产奶量持续下降,乳头坏死,进而被淘汰。乳房炎也是哺乳期妇女常患的疾病,传统的抗生素治疗损伤新生儿的神经系统。在乳房炎的治疗方法中,抗生素因其大量的负面作用使得其已不适合在乳房炎治疗中滥用,应加快开发新的且有效的抗生素替代物用以治疗或预防乳房炎。益生菌在多种疾病的预防与治疗中都有成功的应用实例。因此,益生菌在乳房炎的治疗与预防中是否真的有效是一个有趣的研究方向。益生菌主要于宿主的肠道中发挥其关键作用,因此肠道菌群与乳房炎发病是否有关联,是益生菌能否应用于乳房炎的治疗中需要解答的首个问题。通过菌群移植,对哺乳期小鼠的乳腺组织以及身体其他各项免疫指标进行监控,探究移植健康奶牛粪便小鼠组与移植乳房炎奶牛粪便小鼠组以及移植乳房炎奶牛粪便同时进行益生菌干预组小鼠的乳腺组织是否发生炎症以及炎症程度如何。奶牛肠道菌群移植入无菌小鼠之后,这种菌群结构与功能之上的差异同时被放大形成鲜明的对比。表明健康的肠道菌群结构对于保持奶牛的健康状态有直接的影响,益生菌可调节奶牛的肠道菌群,对于维持奶牛健康,预防和缓解乳房炎有不可忽视的潜能。
[Abstract]:Mastitis is a high incidence disease in dairy cattle farms, which is easy to relapse after antibiotic cure. The repeated attacks of the disease lead to a continuous decline in milk production and nipple necrosis in dairy cows. Then eliminated. Mastitis is also a common disease among lactating women. Traditional antibiotics treat the nervous system of newborns. In the treatment of mastitis. Antibiotics are not suitable for the treatment of mastitis due to their large negative effects. It is necessary to accelerate the development of new and effective antibiotic substitutes for the treatment or prevention of mastitis. Probiotics have been successfully used in the prevention and treatment of many diseases. Whether probiotics are effective in the treatment and prevention of mastitis is an interesting research direction. Probiotics mainly play a key role in the intestinal tract of the host, so whether the intestinal flora is associated with mastitis. It is the first question whether probiotics can be used in the treatment of mastitis. The mammary gland tissue and other immune indexes of lactating mice are monitored by microflora transplantation. To investigate whether the breast tissue of the healthy cow faecal mice group, the transplant mastitis cow faecal mice group and the mammitis cow fecal transplant group with probiotic intervention group had inflammation and the degree of inflammation. Bovine intestinal flora was transplanted into sterile mice. The difference between the structure and function of the bacteria was magnified in sharp contrast, which indicated that the healthy intestinal flora structure had a direct effect on keeping the dairy cows healthy, and the probiotics could regulate the intestinal flora of the cows. It has the potential to maintain cow health, prevent and alleviate mastitis.
【作者单位】：内蒙古农业大学乳品生物技术与工程教育部重点实验室;
【分类号】：S858.23
【正文快照】： hepingdd@vip.sina.com奶牛乳房炎是由挤奶操作时的机械刺激与病原微生物感染所导致的乳腺组织炎症,奶牛患乳房炎后的表现主要为体温升高,乳房红肿、疼痛,乳房腺泡损伤,炎症细胞浸润,腺泡导管水肿,间质出血[1]。对乳房炎研究的技术手段逐步更新,从传统的生物学方法到近年来的

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