禽白血病病毒J亚群感染DF-1细胞后错配修复相关基因转录水平变化的研究

发布时间：2018-01-22 18:33

本文关键词： 禽白血病病毒错配修复 MMR p BRCA　出处：《中国预防兽医学报》2017年03期 　论文类型：期刊论文

【摘要】：为研究禽白血病病毒J亚群(ALV-J)感染DF-1细胞后错配修复相关基因MMR、BRCA1和p53mRNA的转录水平变化,本研究将ALV-J NX0101株接种DF-1细胞,感染7 d后检测ALV-p27抗原,确定病毒感染是否成功。然后提取细胞中总RNA,采用荧光定量PCR以β-actin基因作为内参对MMR(MSH2、MSH3、MLH1和PMS1)、BRCA1和p53基因的mRNA转录情况进行检测。结果表明,与正常对照细胞比较,病毒感染细胞中的MMR(MSH2、MSH3、MLHl和PMSl)和p53基因的mRNA转录水平升高,而BRCA1基因的mRNA转录水平降低。表明在ALV感染宿主细胞后MMR(MSH2、MSH3、MLHl和PMS)、BRCA1和p53参与了宿主细胞DNA损伤的识别及修复过程,这一结果为ALV的致瘤机理的研究提供了新的思路。
[Abstract]:To investigate the transcriptional changes of MMR-BRCA1 and p53 mRNA in DF-1 cells infected with avian leukemia virus subgroup J (ALV-J). In this study, the ALV-J NX0101 strain was inoculated with DF-1 cells, and the ALV-p27 antigen was detected after 7 days of infection to determine whether the virus infection was successful or not. Then the total RNA was extracted from the cells. The 尾 -actin gene was used as the internal reference for MMR-MSH2mH3MH3MLH1 and PMS1) by fluorescence quantitative PCR. The mRNA transcripts of BRCA1 and p53 genes were detected. The results showed that the MMR-MSH2MSH3 in the infected cells was higher than that in the normal control cells. MLHl and PMSL) and p53 gene mRNA transcription level increased, while BRCA1 gene mRNA transcription level decreased, suggesting that MMR(MSH2 after ALV infection of host cells. MSH3MLHl, PMSMS-BRCA1 and p53 are involved in the recognition and repair of DNA damage in host cells, which provides a new idea for the study of tumorigenic mechanism of ALV.
【作者单位】：南京农业大学动物医学院;
【基金】：江苏省自然基金(BK20130686) 国家自然科学基金(308784)
【分类号】：S852.65
【正文快照】： 错配修复(Mismatch repair,MMR)系统广泛存在于从原核到真核生物体中,是细胞分裂增殖过程中的一种保守的自我修复机制,该系统能够特异的识别和修复DNA复制过程中出现的碱基错配、插入和缺失等,从而起到维持整个基因组稳定,降低自发突变的功能,同时也是防止肿瘤发生的重要机制[

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