林麝粪便细菌多样性研究

发布时间：2018-01-22 20:10

本文关键词： 林麝菌群结构 OTU稀释曲线共享菌群　出处：《中国农业大学学报》2016年02期 　论文类型：期刊论文

【摘要】：为研究林麝粪便细菌的结构与组成,选取6只2~3岁的圈养条件相同且健康的公林麝,饲喂精粗比为2∶8的饲粮,收集其新鲜粪便,提取其总DNA,然后用16SrRNA细菌通用引物进行PCR扩增,并用Illumina Miseq250PE测序平台,对扩增子进行Miseq双端测序。结果显示:1)借助Illumina Miseq250PE测序平台对样品进行测序,共获得85 091条有效序列,5 133个有效OTU。2)所得OTU经物种注释被归类为23个门,其中厚壁菌门(Firmicutes)(51.1%±19.9%)、拟杆菌门(Bacteroidetes)(35.6%±15.5%)、变形菌门(Proteobacteria)(5.2%±5.6%)和未分类菌门(*Unassigned)(2.2%±1.0%)为优势菌门,其总和达到菌群相对含量的94.1%;将其进一步分为240个菌属,其中18个为共享菌属,拟杆菌目(*Bacteroidales)(16.5%±10.2%)、瘤胃菌科(*Ruminococcaceae)(14.7%±13.8%)和梭菌目(*Clostridiales)(13.7%±3.9%)含量相对较高。3)OTUs稀释曲线均未达到平台期,说明测序深度不足以覆盖所有的菌群。4)非加权聚类图(PUGMA)显示样品B和E、C和F的菌群多样性相似度较高,而样品A和其他样品的相似度相对较低。由此可见,在正常状态下,健康林麝粪便分布有23个门类细菌群落,以Firmicutes、Bacteroidetes和Proteobacteria为优势菌群,进一步将林麝粪便细菌群落细分为240个属且发现林麝个体间菌群差异性较小且与其他草食动物优势菌群结构相似。
[Abstract]:In order to study the structure and composition of faecal bacteria of musk deer, six male musk deer aged 2 to 3 years old in captivity were selected and fed with a diet of 2: 8. The fresh feces were collected and the total DNA was extracted. Then 16s rRNA bacterial universal primers were used for PCR amplification and Illumina Miseq250PE sequencing platform. Miseq biterminal sequencing of the Amplifier was carried out. The results showed that the sample was sequenced by using Illumina Miseq250PE sequencing platform. A total of 85 091 valid sequences of 5 133 valid OTU. 2 were obtained. The OTU was classified as 23 by species annotation. Among them, 51.1% 卤19.9% of the phylum Firmicutesus and 35.6% 卤15.5% of Bacteroides sp.). Proteobacteria amurensis (5.2% 卤5.6%) and Unassignedae (2.2% 卤1.0%) were the dominant phylum. The sum of them reached 94.1% of the relative content of the flora. It was further divided into 240 genera, 18 of which were shared genera. Bacteroidalesus was 16.5% 卤10.2%. (Ruminococcaceaeae 14.7% 卤13.8.) and Clostridial (13.7% 卤3.9%). The dilution curve of OTUs with relatively high content did not reach the plateau stage. The results indicated that the sequencing depth was not enough to cover all the bacteria. 4) the unweighted cluster map (PUGMA) showed that samples B, EPC and F had higher similarity of flora diversity. The similarity between sample A and other samples was relatively low. It can be seen that under normal condition there are 23 species of bacteria communities in the feces of healthy forest musk deer with Firmicutes. Bacteroidetes and Proteobacteria were the dominant flora. The faecal bacterial community was further subdivided into 240 genera, and it was found that there was little difference among individuals and similar to the dominant flora structure of other herbivorous animals.
【作者单位】：四川农业大学动物营养研究所/动物抗病营养教育部重点实验室;四川省养麝研究所;
【基金】：四川省科技厅国际合作项目(2013HH0043)
【分类号】：S858.94
【正文快照】： 林麝(Moschus berezovskii),又名香獐、林獐、麝獐等,是重要的东亚特产动物,隶属麝科麝属[1],主要分布于中国西南部,是我国一级重点野生保护动物[2]。林麝所产麝香,具有很高的药用价值,被誉为“软黄金”[3],从20世纪60年代起我国便开始进行人工林麝养殖。但到目前为止,林麝的

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