梅花鹿YWHAE基因编码区全长cDNA的克隆及序列分析

发布时间：2018-01-22 21:47

  本文关键词： 梅花鹿 YWHAE基因 --蛋白 克隆 序列分析　出处：《农业生物技术学报》2017年05期 　论文类型：期刊论文

【摘要】：YWHAEA(assignment of the human 14-3-3 epsilon isoform)基因编码14-3-3ε蛋白,此蛋白在发育的生茸区骨膜中特异性高表达。为了克隆梅花鹿(Cervus nippon)YWHAE基因编码区序列,预测其蛋白结构和功能,并研究其在梅花鹿发育过程中的表达特性。本研究以梅花鹿生茸区骨膜为材料,采用RT-PCR技术克隆梅花鹿YWHAE基因编码区序列,利用生物信息学手段预测其结构与功能。实验结果表明,梅花鹿YWHAE基因编码区全长768 bp(Gen Bank No.KY797653),编码255个氨基酸,氨基酸水平上与牛(Bos taurus)相似性高达99.6%;氨基酸序列分析表明,鹿YWHAE基因编码的14-3-3蛋白相对分子量为29 163.86 D,主要定位在细胞质内,属于水溶性蛋白;预测鹿14-3-3蛋白具有19个磷酸化位点、不存在糖基化位点;该蛋白的二级结构主要为螺旋结构,其三级结构呈现出递进螺旋状态。预测14-3-3蛋白的结构特点,本研究为探究14-3-3蛋白与其他蛋白的相互作用提供理论依据,同时为进一步研究鹿茸发育的调控机理提供基础依据。
[Abstract]:YWHAEA(assignment of the human 14-3-3 epsilon isoformin gene encodes 14-3-3 蔚 protein. In order to clone the coding region of Cervus nippon)YWHAE gene from sika deer, the protein structure and function were predicted. In this study, we used the periosteum of sika deer to clone the sequence of YWHAE gene coding region of sika deer by using RT-PCR technique. Bioinformatics was used to predict its structure and function. The results showed that the full-length YWHAE coding region of sika deer was 768 bp(Gen Bank no. KY797653). The amino acid encoding 255 amino acids is similar to Bos taurus at amino acid level. Amino acid sequence analysis showed that the relative molecular weight of 14-3-3 protein encoded by deer YWHAE gene was 29163.86D, which was mainly located in cytoplasm and belonged to water-soluble protein. It was predicted that deer 14-3-3 protein had 19 phosphorylation sites and no glycosylation sites. The secondary structure of the protein is mainly helical structure, and its tertiary structure shows a progressive helical state. The structural characteristics of 14-3-3 protein are predicted. This study provides a theoretical basis for exploring the interaction between 14-3-3 protein and other proteins, and provides a basis for further study on the regulation mechanism of velvet antler development.
【作者单位】： 中国农业科学院特产研究所;
【基金】：中国梅花鹿基因组计划(No.20140309016YY) 中国农业科学院中央级公益性科研院所基本科研业务费专项(No.Y2016PT46和No.1610342016001) 吉林省科技发展计划项目(No.20170307007YY)
【分类号】：S825
【正文快照】： 鹿茸是雄性鹿科动物的第二性征,是唯一可以完全再生的哺乳动物附属器官(Fennessy,1984)。青春期,仔公鹿(Cervus nippon)额外脊上的生茸区骨膜(antlerogenic periosteum,AP)开始发育形成角柄和初角茸,此为公鹿生长出的第一茬茸(Hartwig,Schrudde,1974)。研究已经证实,鹿额外脊，

本文编号：1455812