茴香霉素抗贾第虫的作用研究

发布时间：2018-01-23 09:05

本文关键词： 茴香霉素贾第虫体内体外　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：十二指肠贾第鞭毛虫(Giardia duodenalis),简称贾第虫(Giardia),曾被称为蓝氏贾第虫(Giardia lamblia)。贾第虫是一种重要的人兽共患原虫病病原,主要寄生在人、犬、猫、家畜动物的肠道,可引起腹泻、腹痛、消化不良以及体重下降。贾第虫在我国相当流行,我国城市供水水质和生活饮用水卫生标准均把贾第虫检测作为必检指标。目前,临床治疗贾第虫的药物主要有甲硝唑、替硝唑以及阿苯哒唑等[1],均不同程度上存在副作用和耐药性等问题,研发治疗贾第虫病的新药仍是热点问题。茴香霉素是从浅灰色链霉菌分离的一种抗生素,对阴道毛滴虫、阿米巴原虫等具有一定的杀灭作用,但未见关于茴香霉素对贾第虫作用的报道。本研究拟通过体外试验和体内试验观察茴香霉素的抗贾第虫作用,以期为贾第虫新药的研发提供理论依据和技术支持。茴香霉素体外抗贾第虫的作用研究本研究观察了不同浓度茴香霉素对贾第虫滋养体生长的影响,当茴香霉素浓度为5μg/ml,贾第虫滋养体数量减少97.3%。茴香霉素的最低抑制浓度为0.0167μg/ml。利用扫描电镜观察了茴香霉素(0.5μg/ml)对贾第虫滋养体形态,发现经茴香霉素处理后,滋养体表面凹凸不平,表面细胞膜有一定程度的破裂,内容物出现外溢。茴香霉素对贾第虫感染小鼠的治疗作用研究通过人工感染小鼠1×105个贾第虫包囊,药物治疗试验发现茴香霉素浓度为10 mg/kg,小鼠体内贾第虫滋养体数量减少30%,其平均体重超过阳性对照组。肠道组织切片观察发现药物剂量组病理变化轻微,与阴性对照组相近。阳性对照组出现肠绒毛结构破损及炎性浸润。通过Elisa法检测肠系膜淋巴结的细胞因子水平发现,药物组均高于阳性对照组。茴香霉素对贾第虫感染犬的治疗作用研究通过人工感染犬5×106个贾第虫包囊,进行药物治疗发现茴香霉素剂量为10 mg/kg时,犬体内的贾第虫滋养体数量减少45%。茴香霉素剂量组的试验犬平均增重率超过贾第虫阳性对照组。肠道组织切片观察发现药物剂量组病理变化轻微,与阴性对照组相近,而阳性对照组出现肠绒毛结构破损。
[Abstract]:Giardia duodenalisa (Giardia giardia). Giardia lamblia, once known as Giardia lamblia, is an important zoonotic parasite that parasitizes the intestines of humans, dogs, cats, and domestic animals. It can cause diarrhea, abdominal pain, indigestion and weight loss. Jiardia is very popular in our country. The main drugs for the treatment of giardia are metronidazole, tinidazole and albendazole. [1. There are some side effects and drug resistance in different degree. The research and development of new drug for giardiosis is still a hot issue. Anisomycin is an antibiotic isolated from Streptomyces grayrifolia, which is resistant to Trichomonas vaginalis. Amoeba protozoa has certain killing effect, but there is no report on the effect of anisomycin on giardia. This study aims to observe the anti-giardia effect of anisomycin through in vitro and in vivo experiments. In order to provide theoretical basis and technical support for the research and development of Giardia lamblia in vitro the effects of anisomycin on the growth of Giardia japonica trophozoites were studied. When anisomycin concentration was 5 渭 g / ml. The number of trophozoites of Giardia decreased by 97.3.The minimum inhibitory concentration of anisomycin was 0.0167 渭 g / ml. A scanning electron microscope was used to observe anisomycin 0.5 渭 g / ml. The morphology of trophozoites of Giardia japonica. It was found that the surface of trophozoites was uneven and the cell membrane of trophozoites was ruptured to a certain extent after anisomycin treatment. The therapeutic effect of anisomycin on the mice infected with Giardia lamblia was studied through artificial infection of 1 脳 105 giardia cyst. The drug therapy test showed that the concentration of fengiomycin was 10 mg / kg and the number of Giardia trophozoites in mice decreased by 30%. The average body weight of the drug group was higher than that of the positive control group. The structure of intestinal villi was damaged and inflammatory infiltration was found in the positive control group. The cytokine level of mesenteric lymph nodes was detected by Elisa method. The therapeutic effect of anisomycin on Giardia japonica infected dogs was studied by artificial infection of 5 脳 106 giardia cyst. It was found that the dosage of anisomycin was 10 mg/kg. The number of giardia trophozoites in dogs was decreased by 45%. The average weight gain rate of the dogs in the anisome group was higher than that in the positive control group. The pathological changes were slight in the drug dose group. It was similar to the negative control group, but the intestinal villus structure was damaged in the positive control group.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S859.796

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