鸭疫里氏杆菌porin基因的生物信息学分析

发布时间：2018-01-23 11:15

本文关键词： 鸭疫里氏杆菌微孔蛋白克隆生物信息学分析二级结构抗原性　出处：《黑龙江畜牧兽医》2017年09期 　论文类型：期刊论文

【摘要】：为了研究鸭疫里氏杆菌微孔蛋白(Porin)的结构与功能,试验采用PCR方法克隆了porin基因,并进行了生物信息学分析。结果表明:8种血清型的鸭疫里氏杆菌均在1 200 bp处获得目的片段,BLAST分析显示,8种血清型菌株的porin基因与ATCC 11845等菌株的同源性均达到100%;二级结构预测显示,Poirn蛋白前端存在3个α螺旋,为蛋白的信号肽部分,其余为β折叠和无规则卷曲;三级结构预测显示,Porin蛋白为8个β折叠所构成的圆桶结构,符合革兰氏阴性菌微孔蛋白的结构特点;抗原性分析显示,Porin蛋白的5个区段可能存在B细胞抗原表位。说明Poirn蛋白在鸭疫里氏杆菌菌株间具有高度同源性,且具有潜在B细胞抗原表位,Porin蛋白可用于鸭疫里氏杆菌的鉴定和疫苗的研发。
[Abstract]:In order to study the structure and function of microporin (Porin), porin gene was cloned by PCR method. Bioinformatics analysis was carried out. The results showed that the target fragment blast was obtained at 1 200 BP in 8 serotypes of Leucella duck. The homology of porin gene between 8 serotype strains and ATCC 11845 was 100%. The prediction of the secondary structure showed that there were three 伪 helices in the front end of Poirn protein, which were the signal peptide of the protein, while the others were 尾 -folded and irregularly curled. The tertiary structure prediction showed that the Porin protein was a barrel composed of 8 尾 folds, which was in line with the structural characteristics of micropore protein of Gram-negative bacteria. The antigenicity analysis showed that B cell epitopes might exist in five regions of Porin protein, which indicated that Poirn protein had high homology among the strains of Leucella Duck. The Porin protein with potential B cell antigen epitope can be used in the identification of Riemiella Duck and the development of vaccine.
【作者单位】：吉林农业大学动物科学技术学院;中国兽医药品监察所;吉林农业大学中药材学院;吉林农业大学教研基地管理处;
【基金】：吉林省教育厅“十三五”科学技术研究项目(2016192) 吉林农业大学博士启动基金项目(201405)
【分类号】：S852.61
【正文快照】： 教研基地管理处,长春130118)鸭传染性浆膜炎是由鸭疫里氏杆菌(Riemerellaanatipestifer,RA)引起的雏鸭的一种急性或慢性、败血性传染病,是当前危害养鸭业的最重要细菌病之一[1]。RA的血清型多达21种,各血清型之间缺少交叉保护作用,给该病的免疫和快速诊断带来困难[2]。外膜微

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