辽宁地区奶牛乳房炎源大肠杆菌毒力基因及耐药基因检测分析

发布时间：2018-01-23 11:27

本文关键词： 奶牛乳房炎大肠杆菌毒力基因耐药基因　出处：《中国畜牧兽医》2017年06期 　论文类型：期刊论文

【摘要】：为了掌握辽宁地区规模奶牛场乳房炎源大肠杆菌携带的毒力基因和耐药基因,为奶牛养殖业提供更好的乳房炎防制方案,本研究采用PCR检测方法对辽宁地区多个规模奶牛场临床奶牛乳房炎奶样中分离的66株大肠杆菌进行了4种毒力基因和4种耐药基因的检测分析。结果发现,66株大肠杆菌中仅有1株未检出相关目的基因,其余65株中最少检出2种目的基因,最多检出7种目的基因。其中,毒力基因stx2e、eaeA、K99和astA的检出率分别为56.1%、47.0%、34.8%和31.8%,双重毒力基因的检出率达到43.9%,以eaeA/stx2e基因型的检出率最高;耐药基因sul3、sul1、cmlA及aacA4的检出率分别为87.9%、83.3%、40.9%和28.8%,双重耐药基因的检出率为36.4%,以sul1/sul3基因型检出率最高;三重耐药基因的检出率为37.9%,以cmlA/sul1/sul3检出率最高。本研究结果证实,辽宁地区奶牛乳房炎源大肠杆菌携带磺胺类耐药基因和氯霉素类耐药基因的比率较高,与大肠杆菌的耐药性有较直接的关系,该结果对于辽宁地区奶牛乳房炎的防制具有重要的指导意义,更具有重要的公共卫生意义。
[Abstract]:In order to grasp the virulence gene and drug resistance gene carried by E. coli in large scale dairy farm in Liaoning province, a better scheme of mastitis prevention and control was provided for dairy cattle breeding industry. In this study, 66 strains of Escherichia coli isolated from clinical dairy cow mastitis milk samples in Liaoning area were detected by PCR method. The results showed that there were 4 virulence genes and 4 drug resistance genes. . Only one of 66 Escherichia coli strains had not detected the related target gene, while the remaining 65 strains had at least 2 target genes and 7 target genes. Among them, stx2eeaeA was the virulence gene. The detection rates of K99 and astA were 47.8% and 31.8%, respectively. The detection rate of double virulence gene reached 43.9%. The detection rate of eaeA/stx2e genotype was the highest. The detection rates of cmlA and aacA4 were 87.9% and 28.8%, respectively. The detection rate of double drug resistance gene was 36.4%, with sul1/sul3 genotype being the highest. The detection rate of triple drug resistance gene was 37. 9%, and the detection rate of cmlA/sul1/sul3 was the highest. The ratio of sulfadiamide-resistant genes and chloramphenicol resistant genes was higher in dairy cow mastitis source Escherichia coli in Liaoning area, which was directly related to the resistance of Escherichia coli. The results have important guiding significance for the prevention and control of dairy cow mastitis in Liaoning area, and also have important public health significance.
【作者单位】：辽宁省动物医学研究院;辽宁省动物疫病预防控制中心;辽宁益康生物股份有限公司;中国兽医药品监察所;
【基金】：辽宁省博士启动基金(201501119)
【分类号】：S852.61
【正文快照】： 乳房炎是一种在泌乳盛期和乳产量高的成年奶牛中多发的疾病,该病主要的临床表现为乳区红、肿、热、痛及泌乳障碍,严重制约了乳产业的健康发展。引起奶牛乳房炎的因素众多,如遗传因素、饲养管理因素等,但病原微生物感染是其最主要的病因[1-2]。目前,抗菌药物仍是临床奶牛乳房炎

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