泰妙菌素生产菌种原生质体制备条件优化

发布时间：2018-01-25 07:52

  本文关键词： 泰妙菌素 原生质体 溶壁酶　出处：《安徽农业科学》2016年35期 　论文类型：期刊论文

【摘要】：[目的]探讨泰妙菌素生产菌种原生质体的最优制备方法。[方法]通过对原生质体制备条件进行优化,建立泰妙生产菌种原生质体制备体系。[结果]优化后制备条件:以培养3 d的菌丝体为材料,将其置于含质量浓度0.10%溶壁酶、0.60 mol/L MgSO_4的酶液中,25℃酶解3.0~3.5 h,将酶解液用G2砂芯漏斗过滤,滤液即为原生质体悬液。采用该方法,泰妙生产菌种原生质体制备率达1.5×10~6个/m L。[结论]试验结果为提高泰妙菌素生产菌种的产量提供了理论依据。
[Abstract]:[Objective] to study the optimal preparation method of protoplasts for the production of Tymiao mycin. [Methods the protoplast preparation system was established by optimizing the preparation conditions of protoplasts. [Results: the optimized preparation conditions were as follows: the mycelium was cultured for 3 days and placed in the enzyme solution containing 0.10% wall-lysozyme and 0.60 mol/L MgSO_4 by mass concentration. Enzymatic hydrolysis at 25 鈩，

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