枯草芽孢杆菌发酵金樱子的工艺优化

发布时间：2018-01-25 12:10

本文关键词： 枯草芽孢杆菌金樱子饲料添加剂发酵工艺优化　出处：《中国畜牧兽医》2017年01期 　论文类型：期刊论文

【摘要】：试验旨在研究枯草芽孢杆菌发酵金樱子饲料添加剂的最佳工艺。通过单因素和响应面相结合的试验方法对枯草芽孢杆菌发酵金樱子生产工艺进行优化。选用枯草芽孢杆菌对中草药金樱子进行发酵,以金樱子多糖含量为检测指标,在碳源、氮源、含水量、接种量和发酵时间5个单因素的基础上选择对发酵效果影响较大的关键因素,进行响应面试验来进一步优化。结果表明,5个单因素中,含水量、接种量和发酵时间对发酵效果影响较大,为关键因素,经响应面法进一步优化后得出最佳条件为:含水量为53.79%、接种量为5.74%、发酵时间为2d,此条件下测得金樱子多糖平均值为15.47%,与响应面预测值15.36%接近。相较于未发酵金樱子中10.49%的金樱子多糖,发酵后金樱子多糖提高了47.47%。此发酵工艺简单易操作,发酵效果显著,可为安全绿色饲料添加剂的研制提供理论依据。
[Abstract]:The purpose of the experiment was to study the optimum technology of fermentation of goldenberry seed feed additive by Bacillus subtilis. The production process of Bacillus subtilis fermentation was optimized by combining single factor and response surface. Bacillus spp., a Chinese herbal medicine, was fermented by Bacillus spp. On the basis of five single factors, carbon source, nitrogen source, water content, inoculation amount and fermentation time, the key factors affecting the fermentation effect were selected. The results showed that the water content, inoculation amount and fermentation time had a great influence on the fermentation effect, and the key factors were water content, inoculation amount and fermentation time. The optimum conditions were as follows: water content was 53.79, inoculation amount was 5.74, fermentation time was 2 days, and the average value of polysaccharides was 15.47%. Compared with the 10.49% polysaccharides from unfermented goldenberry, the polysaccharides increased by 47.47. this fermentation process is simple and easy to operate. The effect of fermentation is remarkable, which can provide theoretical basis for the development of safe green feed additive.
【作者单位】：东北农业大学动物科学技术学院;高唐六和荣达饲料有限公司;
【基金】：国家科技支撑计划(2014BAD13B03-1)
【分类号】：S816.7
【正文快照】： 抗生素类饲料添加剂在给畜牧生产带来经济效益的同时所带来的负面效应越来越受到人们的关注,这便迫使人们去寻找一种新型的绿色、安全的饲料添加剂来满足畜牧生产的需求[1]。在中国,中草药作为饲料添加剂应用于动物生产中已具有悠久的历史,其毒副作用小,在肉、蛋、奶等产品中

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