产β-葡萄糖苷酶芽孢杆菌降解豆粕的发酵条件优化及抗营养因子测定

发布时间：2018-01-25 13:39

本文关键词： β-葡萄糖苷酶发酵培养基优化抗营养因子　出处：《饲料工业》2017年14期 　论文类型：期刊论文

【摘要】：试验优化得到了降解豆粕芽孢杆菌Z-1生长的最优发酵条件,并对发酵后豆粕中的抗营养因子进行了测定。采用单因素试验的方法在摇床培养的基础上对β-葡萄糖苷酶芽孢杆菌的生长主要影响因素进行了考察,其中包括发酵培养基的C源、N源、无机盐、p H值、接种量、发酵时间等。通过设计正交试验对各因素的浓度和组合以及最佳培养条件进行了优化,得到该菌株产酶的最适条件为:糊精5.0%、蛋白胨1.0%、Cu SO4·5H2O 0.1%、Fe SO4·7H2O 0.01%、接种量3%、p H值为7、培养时间72 h。用优化后的培养基进行发酵,在产酶活性上比优化前提高了35.5%,抗营养因子数量下降了61.6%。
[Abstract]:The optimum fermentation conditions for the growth of Bacillus sojae Z-1 were obtained. The main factors affecting the growth of 尾-glucosidase bacillus were investigated by single factor test on the basis of shaker culture. It includes C source N source, inorganic salt pH value, inoculation amount, fermentation time and so on. The concentration and combination of each factor and the optimum culture conditions were optimized by orthogonal design. The optimum conditions for enzyme production of the strain were as follows: dextrin 5.0, peptone 1.0 and Cu SO4 路5H2O 0.1 and Fe SO4 路7H2O 0.01. The inoculation amount was 3%. The pH value was 7 and the culture time was 72 hours. The enzyme production activity was increased by 35.5and the amount of anti-nutrition factor was decreased by 61.6% compared with that before optimization.
【作者单位】：河北农业大学生命科学学院;新疆师范大学地理科学与旅游学院;
【基金】：国家星火项目[2010GA620010] 河北省农业科技成果转化资金项目[09825125D]
【分类号】：S816.6
【正文快照】： 豆粕是畜牧养殖业中使用最为广泛的一种优质植物性蛋白源。豆粕的营养成分主要有蛋白质(40%~44%)、脂肪(1%~2%)、碳水化合物(10%~15%);另外,豆粕中含有多种矿物质、维生素和必需氨基酸,营养成分比较齐全且种类均衡;此外,豆粕中还含有异黄酮、磷脂等生物活性物质,是饲料业中应

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