福建省羊传染性脓疱病毒F1L、B2L和VIR基因的遗传变异分析

发布时间：2018-01-27 02:28

本文关键词： 羊传染性脓疱病毒 FL基因 BL基因 VIR基因变异分析　出处：《动物医学进展》2017年02期 　论文类型：期刊论文

【摘要】：为探明2014年-2015年在福建省流行的羊传染性脓疱病毒(ORFV)遗传变异情况,对10株ORFV流行毒株的F1L、B2L和VIR基因进行克隆、测序及分析。结果表明,10株ORFV F1L基因之间的核苷酸序列同源性为97.6%~100%,与国内株的核苷酸序列同源性为96.8%~99.7%,与NZ2参考株的核苷酸序列同源性为96.3%~97.1%;同NZ2参考株比较,FJ-YT2014缺失2个氨基酸;10株ORFV B2L基因之间核苷酸序列同源性为97.5%~99.9%,与国内株的核苷酸序列同源性为96.7%~99.5%,与NZ2参考株的核苷酸序列同源性为96.7%~97.7%;10株ORFV VIR基因之间的核苷酸序列同源性为95.8%~99.5%,与国内株的核苷酸序列同源性为94.6%~99.6%,与NZ2参考株的核苷酸序列同源性为94.6%~96.4%。基于基因核苷酸序列的遗传进化分析表明,10株ORFV F1L基因与福建省分离株、山西株和新疆株亲缘关系较近;10株ORFV B2L基因与新疆、山西、德国毒株亲缘关系较近;10株ORFV VIR基因与台湾、新疆株亲缘关系较近。结果提示,当前福建省流行的ORFV F1L、B2L和VIR基因尚未出现明显变异,但是其F1L、B2L和VIR基因核苷酸序列之间普遍存在异质性。
[Abstract]:In order to investigate the genetic variation of infectious pustular virus (ORFV) from 2014 to 2015 in Fujian Province, the F1L of 10 strains of ORFV were studied. B2L gene and VIR gene were cloned, sequenced and analyzed. The results showed that the nucleotide sequence homology between ORFV F1L gene and B2L gene was 97.6%. The homology of nucleotide sequence with domestic strain was 96.8and 99.70.The homology with NZ2 reference strain was 96.3and 97.1; Compared with NZ2 reference strain, FJ-YT2014 lacks 2 amino acids. The homology of nucleotide sequence between 10 strains of ORFV B2L gene and domestic strain was 97.5% and 99.9%, respectively. The nucleotide sequence homology with NZ2 reference strain was 96.7%. The nucleotide sequence homology among the 10 strains of ORFV VIR gene was 95.88.99. 5%, and the homology of nucleotide sequence with domestic strains was 94.6%. The nucleotide sequence homology with NZ2 reference strain was 94.6%. The genetic evolution analysis based on the nucleotide sequence showed that ORFV F1L gene of 10 strains and Fujian Province isolate. The relationship between Shanxi plant and Xinjiang plant is close; 10 strains of ORFV B2L gene were closely related to Xinjiang, Shanxi and Germany. 10 strains of ORFV VIR gene were closely related to those in Taiwan and Xinjiang. The results suggested that there was no significant variation in the ORFV F1L LU B2L and VIR genes in Fujian Province at present. But the nucleotide sequence of F 1 L B 2 L and VIR gene is heterogeneity.
【作者单位】：福建省农业科学院畜牧兽医研究所;
【基金】：福建省科技计划项目(2014R1102) 福建省公益性科研专项(2014R1101002-8)
【分类号】：S852.654
【正文快照】： 羊传染性脓疱(Orf)是由羊传染性脓疱病毒(Orf virus,ORFV)引起的羊接触性、嗜上皮性、人兽共患的传染病[1],各个年龄段的羊群都能感染发病,主要临床症状表现为嘴唇、口腔黏膜、外阴等出现丘疹或水疱,其中对羔羊的危害最严重。ORFV属于痘病毒科副痘病毒属成员,病毒的核酸为线性

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