肉桂酸衍生物抗PRV及其作用机制研究

发布时间：2018-01-28 09:42

本文关键词： 肉桂酸衍生物猪伪狂犬病病毒抗病毒天然化合物　出处：《中国兽药杂志》2017年04期 　论文类型：期刊论文

【摘要】：以猪伪狂犬病病毒(PRV)感染PK-15细胞,对27种天然化合物的抗PRV作用进行筛查,并探讨抗PRV活性物质的作用机制。分别将27种天然化合物以不同方式作用于感染细胞,采用细胞病变法(CPE)和噻唑蓝比色法(MTT)测定其细胞毒性及对病毒吸附、复制和直接杀灭的作用。在药物与PRV共同作用于PK-15细胞24、48、72 h后,RT-PCR检测PRV的代表基因IE180、UL30和UL22在胞内的表达量。结果显示,肉桂酸衍生物(cinnamic acid derivative,CAD)具有抗PRV的活性,其EC50为(8.443±0.216)μg/m L,SI为6.6,最高抑制率为76%。3个时间点胞内IE180、UL30和UL22基因的拷贝数显著降低(P0.01),且呈剂量依赖性。结果表明,CAD对PRV有直接灭活作用,其作用机制是阻断PRV在细胞内的生物合成过程,但CAD对PRV的吸附过程没有阻断作用。CAD可作为抗PRV的候选药物。
[Abstract]:PK-15 cells were infected with porcine pseudorabies virus (PRV) and the anti-#en1# effects of 27 natural compounds were screened. To explore the mechanism of anti- PRV active substances, 27 natural compounds were treated in different ways in infected cells. Cytopathic assay (CPE) and thiazolyl blue colorimetry (MTT) were used to determine their cytotoxicity and viral adsorption. Replication and direct killing. RT-PCR was used to detect the representative gene IE180 of PRV in PK-15 cells for 72 h after co-treatment with PRV. The results showed that cinnamic acid derived cad) had anti-#en3# activity. Its EC50 was 8.443 卤0.216) 渭 g / mL ~ (-1) SI was 6.6, and the highest inhibitory rate was 76.3 IE180. The copy number of UL30 and UL22 gene decreased significantly in a dose-dependent manner. The results showed that PRV was inactivated directly. The mechanism is to block the biosynthesis of PRV in cells, but the adsorption process of PRV by CAD is not blocked. CAD can be used as a candidate drug to resist PRV.
【作者单位】：山西农业大学动物科技学院;北京中医药大学中药学院;
【基金】：农业科技成果转化资金(2014GB2A3000)
【分类号】：S859.7
【正文快照】： 伪狂犬病是由伪狂犬病病毒(pseudorabiesvirus,PRV)引起的多种家畜和野生动物以发热、奇痒及脑脊髓炎为特征的急性传染病。伪狂犬病病毒属于疱疹病毒家族中的α-疱疹病毒亚科,该病毒引起的仔猪疾病又称为Aujeszky’s病[1]。猪是伪狂犬病病毒的自然宿主。除此之外,PRV还能够感

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