江苏地区鸡源弯曲杆菌分离鉴定及耐药性研究

发布时间：2018-01-28 09:44

本文关键词： 弯曲杆菌鸡耐药性江苏　出处：《中国家禽》2017年18期 　论文类型：期刊论文

【摘要】：为了解江苏地区鸡源弯曲杆菌的流行和耐药状况,采集养殖场及农贸市场鸡泄殖腔棉拭子样品和超市鸡肉样品进行空肠弯曲杆菌、结肠弯曲杆菌的分离与鉴定,对分离菌株采用纸片扩散法检测其耐药情况。结果显示:泄殖腔棉拭子样品弯曲杆菌总分离率为38.68%(205/530),其中空肠弯曲杆菌和结肠弯曲杆菌阳性率分别为27.36%(145/530)和11.32%(60/530);鸡肉样品中弯曲杆菌总分离率为45.33%(68/150),其中空肠弯曲杆菌和结肠弯曲杆菌阳性率分别为30.00%(45/150)和15.33%(23/150)。所有样品空肠弯曲杆菌分离率极显著高于结肠弯曲杆菌(P0.01),就泄殖腔棉拭子样品而言,养殖场样品弯曲杆菌总分离率显著高于农贸市场样品(P0.05),泄殖腔棉拭子样品与鸡肉样品弯曲杆菌分离率无显著性差异(P0.05)。耐药检测结果显示,弯曲杆菌对9大类27种抗生素耐药率在80%以上的有6种,分别为头孢噻肟、头孢曲松、诺氟沙星、环丙沙星、甲氧苄胺嘧啶和克林霉素,所有菌株表现出多重耐药现象,耐药谱主要集中在12耐、13耐和14耐。研究表明,江苏地区鸡源弯曲杆菌流行普遍,分离菌株存在耐药现象且多重耐药情况较严重。
[Abstract]:In order to understand the prevalence and drug resistance of campylobacter from chickens in Jiangsu province, samples of cloacal cotton swabs from poultry farms and farmers' markets and chicken samples from supermarkets were isolated and identified by Campylobacter jejuni and Campylobacter colon. The results showed that the total isolation rate of Campylobacter in cloacal cotton swabs was 38.68% 20.5 / 530). The positive rates of Campylobacter jejuni and Campylobacter coli were 27.36 / 145 / 530 and 11.32 / 60 / 530 respectively. The total isolation rate of Campylobacter in chicken samples was 45.33%. The positive rates of Campylobacter jejuni and Campylobacter Colon were 30.0045 / 150 and 15.33 / 23 / 150, respectively. The isolation rate of Campylobacter jejuni from all samples was significantly higher than that from Campylobacter coli (P 0.01). As far as cloacal cotton swab samples were concerned, the total isolation rate of Campylobacter in farm samples was significantly higher than that in farmers' market samples (P0.05). There was no significant difference in the isolation rate of Campylobacter between cloacal cotton swabs and chicken samples. Six strains of campylobacter campylobacter with resistance rate above 80% to 27 antibiotics in 9 categories were cefotaxime, ceftriaxone, norfloxacin, ciprofloxacin, methoxybenzylamine and clindamycin. All the strains showed multidrug resistance, and the resistance spectrum mainly focused on resistance 12 and 14. The results showed that campylobacter from chicken was prevalent in Jiangsu province. The isolated strains were resistant to drugs and the situation of multiple drug resistance was serious.
【作者单位】：江苏省家禽科学研究所;农业部家禽品质监督检验测试中心(扬州);
【基金】：扬州市社会发展项目(yz2016058) 国家农产品质量安全风险评估重大专项(GJFP2017007) 江苏省农业三新工程项目(SXGC[2016]292)
【分类号】：S852.61
【正文快照】： 2.农业部家禽品质监督检验测试中心(扬州),江苏扬州225125)弯曲杆菌是一种细长呈弯曲螺旋状的革兰阴性微需氧菌,是世界范围内引起人细菌性胃肠炎最主要的食源性病原菌。弯曲杆菌中能引起人类感染的主要是空肠弯曲杆菌(Campylobacterjejuni)和结肠弯曲杆菌(Campylobacter coli)

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