聚乙烯亚胺配合灭活禽流感病毒口服免疫对鸡免疫反应和生长发育的影响

发布时间：2018-01-30 09:58

本文关键词： 聚乙烯亚胺口服免疫灭活禽流感病毒　出处：《南京农业大学学报》2017年02期 　论文类型：期刊论文

【摘要】：[目的]本文旨在研究聚乙烯亚胺(PEI)配合灭活H5N1禽流感病毒口服免疫对鸡免疫反应和生长发育的影响。[方法]1日龄雏鸡35只,随机分成5组,每组7只。根据免疫方法分为口服生理盐水0.3 m L组(CK)、口服灭活禽流感病毒0.25 m L组(IAIV)、口服0.08 g聚乙烯亚胺配合0.25 m L灭活禽流感病毒组[IAIV+PEI(0.08)]、口服0.02 g聚乙烯亚胺配合0.25 m L灭活禽流感病毒组[IAIV+PEI(0.02)]和皮下注射灭活禽流感油乳佐剂疫苗组(i H)。分别在首次免疫后第3、5、7周通过检测口腔棉拭子、肠道和气管涮洗液中特异性Ig A、血清中特异性Ig G和HI抗体水平对免疫效果进行评价,然后称体质量,检测空肠和回肠绒毛长度以对生长发育效果进行评价。[结果]0.02 g聚乙烯亚胺配合0.25 m L灭活禽流感病毒口服免疫雏鸡的口腔棉拭子和肠道涮洗液中特异性Ig A水平显著高于单独口服灭活病毒雏鸡组(P0.05);0.08 g和0.02 g聚乙烯亚胺分别配合0.25 m L灭活禽流感病毒口服免疫雏鸡后第7周,血清中特异性Ig G水平极显著升高(P0.01);0.02 g聚乙烯亚胺配合0.25 m L灭活禽流感病毒口服免疫雏鸡后,其空肠、回肠绒毛长度比单独口服灭活病毒极显著增长(P0.01)。[结论]0.02 g聚乙烯亚胺配合0.25 m L灭活H5N1禽流感病毒口服免疫雏鸡,不仅增强免疫反应,提高免疫力,还能促进营养物质吸收。
[Abstract]:[Objective] this paper aims to study the polyethyleneimine (PEI) with inactivated H5N1 avian influenza virus in chicken oral immune immune response and affect the growth and development of]1 method. 35 day old chicks were randomly divided into 5 groups, 7 rats in each group. According to the immune method is divided into oral saline 0.3 M L group (CK), oral inactivated avian influenza virus M 0.25 L group (IAIV), 0.08 g oral polyethyleneimine combined with 0.25 m of L inactivated avian influenza virus group [IAIV+PEI (0.08)], 0.02 g oral polyethyleneimine with 0.25 m L inactivated avian influenza virus group [IAIV+PEI (0.02)] and subcutaneous injection of inactivated flu oil emulsion adjuvant vaccine group (I H) respectively. In 3,5,7 weeks after the first immunization by detecting oral swab, intestinal and tracheal washing fluid in specific Ig A, serum specific Ig G and HI antibody levels to evaluate the immune effect, Ismaeil detection of jejunum and ileum villus length on the growth of hair To evaluate the effect of education. The results of]0.02 g with 0.25 m L and polyethylenimine specific Ig A level of oral swabs and intestinal washing fluid live bird flu virus oral Immunized Chicken was significantly higher than the single oral inactivated virus of chicken group (P0.05); 0.08 g and 0.02 g respectively with 0.25 m polyethylene imine L arc seventh weeks of live bird flu virus after oral immunization of chickens, serum specific Ig G levels were significantly increased (P0.01); 0.02 g 0.25 m with polyethylenimine L inactivated avian influenza virus in chickens after oral immunization, the jejunum, ileum villus length than single oral inactivated virus significantly increased (P0.01). Conclusion]0.02 g 0.25 m L with polyethylenimine inactivated H5N1 avian influenza virus oral immunization of chickens, not only enhanced immune response, enhance immunity, can promote the absorption of nutrients.

【作者单位】：南京农业大学农业部动物生理生化重点开放实验室;
【基金】：国家自然科学基金项目(31172302) 国家公益性行业(农业)科研专项(201303046)
【分类号】：S858.31
【正文快照】： 高致病性禽流感(highly pathogenic avian influenza,HPAI)以高死亡率为主要特征,给养禽业造成严重的经济损失[1]。目前预防和控制禽流感主要依靠皮下注射禽流感全病毒灭活疫苗,但此种免疫方式不能有效刺激局部黏膜免疫的产生,而且皮下注射疫苗还会导致动物因应激反应造成的生

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