PPARγ对猪血管内皮细胞增殖、迁移及小管形成的影响

发布时间：2018-01-30 22:52

  本文关键词： 过氧化物酶体增殖物激活受体γ(PPARγ) 猪 血管内皮细胞　出处：《中国畜牧兽医》2017年09期 　论文类型：期刊论文

【摘要】：试验旨在研究过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptorsγ,PPARγ)对猪血管内皮细胞增殖、迁移及小管形成的影响,并探讨PPARγ在猪体外血管生成中的作用。设置PPARγ激动剂组(5、10、15、20μmol/L罗格列酮)、抑制剂组(5、10、15、20μmol/L T0070907)及对照组,通过iCelligence细胞功能分析、划痕试验和Matrigel基质胶三维培养,构建猪体外血管生成的模型,模拟猪体内血管生成的环境,分别对猪血管内皮细胞的增殖、迁移、小管形成能力进行测定,同时根据NCBI已有的相关序列,应用Primer Premier 5.0软件设计PPARγ基因特异性引物,利用SYBR GreenⅠ实时荧光定量PCR检测PPARγ基因mRNA相对表达量,对PPARγ的体外作用效果进行验证。结果显示,5、10μmol/L罗格列酮能促进猪血管内皮细胞增殖、迁移及小管形成,T0070907的抑制效果在试验浓度区间内(5~15μmol/L)随浓度升高而加强,较高浓度(20μmol/L)的两种药物均由于药物毒性的影响对细胞活动产生干扰。此外,5~20μmol/L罗格列酮和5~20μmol/L T0070907能分别提高和降低PPARγ基因mRNA相对表达量,且浓度趋势与增殖、迁移、小管形成的试验结果一致。综上所述,通过激活PPARγ可以对猪血管内皮细胞的增殖、迁移及小管形成产生促进效果,提示其在猪体外血管生成中具有积极作用,可为研究PPARγ对猪胎盘血管发生的影响提供参考依据。
[Abstract]:The aim of this study was to study peroxisome proliferator-activated receptors 纬-peroxisome receptor. The effects of PPAR 纬 on the proliferation, migration and tubule formation of porcine vascular endothelial cells, and the role of PPAR 纬 in porcine angiogenesis in vitro were investigated. 20 渭 mol/L rosiglitazone and 1520 渭 mol/L T0070907 in the inhibitor group and the control group were analyzed by iCelligence cell function analysis. Scratch test and three-dimensional culture of Matrigel matrix glue were used to construct the model of porcine angiogenesis in vitro, to simulate the environment of pig angiogenesis, and to proliferate and migrate porcine vascular endothelial cells, respectively. The ability of tubule formation was measured and the specific primers of PPAR 纬 gene were designed by using Primer Premier 5.0 software according to the related sequences of NCBI. The relative expression of PPAR 纬 gene mRNA was detected by SYBR Green 鈪，

本文编号：1477450