绒山羊胎儿成纤维细胞和成体干细胞体外生长特性的比较

发布时间：2018-01-31 03:41

  本文关键词： 胎儿成纤维细胞 成体干细胞 多能性 增殖 凋亡　出处：《基因组学与应用生物学》2017年02期 　论文类型：期刊论文

【摘要】：本研究旨在探索阿尔巴斯白绒山羊成体干细胞和胎儿成纤维细胞体外生长、增殖能力和胚胎干细胞特性的差异,为成体干细胞的应用提供新的理论基础。研究以胎儿成纤维细胞(fetal fibroblast cells,FFCs)作为对照,检测阿尔巴斯白绒山羊3种成体干细胞,脂肪间充质干细胞(adipose-derived stem cells,ADSCs)、骨髓间充质干细胞(bone marrow stromal cells,BMSCs)和肌肉卫星细胞(muscle-derived satellite cells,MDSCs)中多能性(NANOG,OCT4,SOX2)、增殖(TERT,PCNA)、凋亡(P53,BAX)基因的转录和表达。表明g ADSCs、g BMSCs和g MDSCs低表达NANOG、OCT4和SOX2,与g FFCs没有明显差异;TERT在g BMSCs和g MDSCs中的表达量高于g FFCs中的表达量,而在g ADSCs中的表达量则低于g FFCs;PCNA和BAX在g FFCs和g MDSCs中高表达,在g ADSCs和g BMSCs中低表达;P53在g BMSCs中的相对表达量较高,在g FFCs、g ADSCs和g MDSCs中的相对表达量均较低。现象表明,在一定程度上成体干细胞的胚胎干细胞特性及增殖能力优于胎儿成纤维细胞。
[Abstract]:The aim of this study was to explore the difference between adult stem cells and fetal fibroblasts in vitro growth, proliferation and characteristics of embryonic stem cells. To provide a new theoretical basis for the application of adult stem cells, fetal fibroblasts (FFCs) were used as control. Three kinds of adult stem cells and adipose-derived stem cells were detected. Bone marrow stromal cells. BMSCs) and muscle-derived satellite cells (MDSCs). The transcription and expression of SOX2, ADSCs, P53 and Bax) genes showed that g ADSCs. G BMSCs and g MDSCs were not significantly different from g FFCs in the expression of NANOGN OCT4 and SOX2. The expression of TERT in g BMSCs and g MDSCs was higher than that in g FFCs, but the expression in g ADSCs was lower than that in g FFCs. The expression of PCNA and BAX was high in g FFCs and g MDSCs, and low in g ADSCs and g BMSCs. The relative expression of p53 in g BMSCs was higher than that in g FFCsG ADSCs and g MDSCs. To a certain extent, the embryonic stem cells of adult stem cells are superior to fetal fibroblasts in the characteristics and proliferation of embryonic stem cells.
【作者单位】： 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室;
【基金】：高产绒量转基因绒山羊新品种培育项目(2014ZX08008-002)资助
【分类号】：S827
【正文快照】： 阿尔巴斯白绒山羊在内蒙古地区具有很高的经1.2免疫细胞荧光检测多能性增殖和凋亡相关基因济价值,其转基因新品种的培育及大规模繁殖不仅的表达对内蒙古自治区的经济发展具有很大的影响,同时免疫细胞荧光检测显示,gF FCs中几乎检测不到对当代畜牧业的发展也具有重大意义。目前，

本文编号：1478093