中国新疆部分地区马泰勒虫感染的差异性及系统发育分析

发布时间：2018-02-03 04:20

本文关键词： 马泰勒虫感染系统发育　出处：《新疆农业科学》2017年11期 　论文类型：期刊论文

【摘要】：【目的】分析新疆南北疆马泰勒虫感染的差异性及地方流行株遗传进化距离,研究马泰勒虫遗传多样性及其感染率。【方法】采自南北疆478份疑似马匹的血样,经马泰勒虫PCR方法检测,分析马泰勒虫感染情况并应用最大似然法(ML)基于18 S rRNA基因的遗传进化树及构建系统发育树。【结果】在所采集的样品中,中国新疆北疆地区马泰勒虫感染阳性率为13.96%(25/179),中国新疆南疆地区马泰勒虫感染阳性率为27.09%(78/299)。印度、南非、西班牙、伊朗等地方株聚为一支,扩增的18S rRNA基因(MF398476、MF398477)与瑞典地方株聚为一支。【结论】中国新疆南疆马泰勒虫感染率高于北疆,南北疆马泰勒虫感染存在显著性差异(P=0.0010.05),在不同年龄段马匹的感染无显著性差异(P0.05);扩增的马泰勒虫阿勒泰、托克逊地方流行株(MF398477、MF398476)与瑞士地方株(KM046918.1)亲缘关系最近。
[Abstract]:[objective] to analyze the difference of Maleylla infection and the genetic and evolutionary distance of endemic strains in north and south Xinjiang. To study the genetic diversity and infection rate of T. equinalis. [methods] 478 blood samples from northern and southern Xinjiang were collected and detected by PCR method. The genetic evolution tree based on the 18s rRNA gene and the phylogenetic tree were analyzed by using the maximum likelihood method (MLL). [results] the phylogenetic tree was constructed in the samples collected. The positive rate of MarTaylor's infection was 13.96% / 179% in northern Xinjiang, and 27.09% / 299% in southern Xinjiang, China. South Africa, Spain, Iran and other local strains clustered into one, amplified 18s rRNA gene MF398476. MF398477) and the local strains of Sweden were clustered into one branch. [conclusion] the infection rate of Maleylla in southern Xinjiang of China is higher than that in northern Xinjiang. There was a significant difference in infection between north and south Xinjiang (P0. 0010.05), but there was no significant difference in infection of horses in different age groups (P0. 05). The phylogenetic relationship between the amplified strains of Altay, Tocson endemic strain MF398477 and Swiss strain KM046918.1) was the most recent.
【作者单位】：新疆农业大学动物医学学院;和静畜牧兽医站;昭苏县畜牧兽医站;
【基金】：国家自然科学基金项目-新疆联合基金重点项目“新疆马梨形虫病流行规律及其优势媒介蜱生物学特性和检测技术的研究”(U1403283) 新疆农业大学研究生科研创新项目课题“马泰勒虫病中西药物效果筛选及治疗”(XJAUGRI2016-009)~~
【分类号】：S858.21
【正文快照】： 0引言【研究意义】马泰勒虫是由硬蜱传播的顶复门血液内寄生虫,主要寄生于马、骡、驴和斑马等动物的网状内皮系统及红细胞内[1]。引起马临床呈现稽留热、贫血、出血、黄染、呼吸困难等症状[2-4],OIE将其列为B类疫病,我国将其列为二类疫病[5]。【前人研究进展】马泰勒虫病呈世

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