由沙门氏菌引起的犊牛腹泻病的实验室诊断及防治

发布时间：2018-02-03 04:02

本文关键词： 犊牛腹泻沙门氏菌诊治　出处：《中国畜牧兽医》2017年08期 　论文类型：期刊论文

【摘要】：为了探究山西某规模化奶牛场犊牛腹泻病的发病原因及相应的综合防治措施,本研究通过对该场犊牛腹泻病发病情况进行调查,同时对可疑病原轮状病毒、冠状病毒、大肠杆菌F5(K99)、小球隐孢子虫、沙门氏菌进行实验室检测,分离并培养了可疑病原菌,并对该病原菌进行形态学和血清学鉴定,选取18种常见抗生素,通过药敏试验筛选对该菌敏感的抗生素。结果显示,经临床诊断和实验室检测基本排除大肠杆菌F5(K99)、轮状病毒、冠状病毒、小球隐孢子虫和球虫感染的可能性,分离的可疑菌具有沙门氏菌的菌落特征和细菌形态,并与沙门氏菌O多价血清A-F呈现阳性反应,确诊该病原主要为沙门氏菌。药敏试验结果显示,所分离的沙门氏菌对头孢曲松、庆大霉素、头孢拉定、阿米卡星等药物高度敏感,可推荐用于临床治疗。结合以上结果,本研究提出该病的具体防治措施,并取得了较好的防治效果。本研究初步为犊牛腹泻病中沙门氏菌病的诊断和防治提供了参考,对犊牛腹泻病病原的早期诊断和治疗具有重要的临床意义。
[Abstract]:In order to investigate the causes of diarrhea of calves in a large scale dairy farm in Shanxi Province and the corresponding comprehensive prevention and treatment measures, this study investigated the incidence of diarrhea disease of calves in this farm, and at the same time made an investigation on the suspected pathogenic rotavirus. Coronavirus, Escherichia coli F5 / K99, Cryptosporidium microphylla and Salmonella were detected in laboratory, the suspected pathogens were isolated and cultured, and the pathogen was identified by morphology and serology. A total of 18 common antibiotics were selected to screen the antibiotics sensitive to the strain by drug sensitivity test. The results showed that Escherichia coli F5K99, rotavirus were basically excluded by clinical diagnosis and laboratory examination. The possibility of infection of coronavirus, Cryptosporidium microphylla and coccidiosis. The isolated suspicious bacteria had colony characteristics and bacterial morphology of Salmonella, and showed positive reaction with O-polyvalent serum A-F of Salmonella. The results of drug sensitivity test showed that the isolated Salmonella was highly sensitive to ceftriaxone, gentamicin, cefradine and amikacin. Can be recommended for clinical treatment. Combined with the above results, this study proposed specific measures for the prevention and treatment of the disease. This study provides a reference for the diagnosis and control of salmonellosis in calf diarrhea and has important clinical significance for the early diagnosis and treatment of the pathogen of diarrhea disease in calves.
【作者单位】：中国农业大学动物医学院;北京市动物疫病预防控制中心;新疆维吾尔自治区动物卫生监督所;
【基金】：国家奶牛产业技术体系(CARS-37) 公益性行业(农业)科研专项经费(201303037)
【分类号】：S858.23
【正文快照】： 犊牛腹泻是由于肠道内细菌、病毒、寄生虫等病原微生物或是营养性因素、环境性因素致使犊牛免疫力低下、脱水、酸中毒、电解质失衡所表现出来的一种综合征兆。该病在全球犊牛中均为常见疾病,多发生于2月龄内的犊牛,严重时可导致70%的犊牛发病,死亡率高达50%[1]。发病犊牛食欲

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