鹅呼肠孤病毒GRV-LA16株的分离鉴定

发布时间：2018-02-04 09:09

本文关键词： 鹅呼肠孤病毒分离鉴定 σC基因　出处：《中国动物传染病学报》2017年03期 　论文类型：期刊论文

【摘要】：为探明安徽省某养鹅场60日龄鹅出现的软脚,排白色粪便,严重者导致鹅死亡的原因,对病死鹅进行剖检,发现其以肝脏肿大,脾脏肿大和坏死以及肺脏充血、出血为主要特征。对分离毒株进行RT-PCR扩增试验、序列分析及动物回归试验,结果表明,该分离毒株为鹅呼肠孤病毒(Goose reovirus,GRV),命名为GRV-LA16株,其ELD50为10-5.7/0.2 m L。病理组织切片结果显示,与对照组相比,肺泡壁胀破融合,毛细血管扩张出血,且肺泡腔有红色丝网状纤维素渗出物。σC基因测序结果分析表明,其与鸭呼肠孤病毒σC基因同源性为87%。GRV-LA16与鸭呼肠孤病毒亲缘关系较近,为同一拓扑群,而与经典的禽呼吸肠孤病毒(Avian orthoreoviruses,ARV)、GRV、番鸭呼肠孤病毒(Muscovy duck reovirus,MDRV)处于不同拓扑群。
[Abstract]:In order to find out the causes of goose death caused by soft feet, excretion of white feces and serious cases in a geese farm in Anhui Province, the sick and dead geese were dissected and found to have liver swelling, splenomegaly and necrosis, as well as congestion of lungs. Haemorrhage was the main characteristic. RT-PCR amplification test, sequence analysis and animal regression test showed that the isolated strain was Goose ReovirusGRV, named as GRV-LA16 strain, and its ELD50 was 10-5.7 / 0.2 mL. Compared with the control group, alveolar wall bulging and fusion, capillary dilatation and hemorrhage, and red silk reticular cellulose exudate were found in alveolar lumen. The homology with the 蟽 C gene of duck reovirus is 87.GRV-LA16 is closely related to duck reovirus and belongs to the same topological group, but it is in different topological groups with classical avian respiratory solitary virus (Avian ARV) and Muscovy duck reovirusMDRV (Muscovy duck reovirusMDRV).
【作者单位】：中国农业科学院上海兽医研究所;
【基金】：国家自然科学基金(31502068) 国家重点研发计划(2016YFD0500800) 上海市科委科技创新(13391901602)
【分类号】：S852.65
【正文快照】： 禽呼肠孤病毒(Avian orthoreoviruses,ARV)为呼肠孤病毒科(Reoviridae)正呼肠孤病毒属(Orthoreovirus)的成员之一,可感染多种禽类[1],包括鸡、火鸡、鸵鸟、水禽、野鸭等禽类,能引起鸡的吸收障碍综合征、腱鞘炎、病毒性关节炎、矮小综合征、免疫抑制等疾病[2,3],现已成为危害养

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