茶皂素对黄羽肉鸡抗炎活性及抗氧化能力的影响

发布时间：2018-02-05 04:59

本文关键词： 茶皂素脂多糖黄羽肉鸡抗炎活性抗氧化　出处：《湖南农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：选用480只1日龄的黄羽肉公鸡,随机分为8个处理,每个处理6个重复,每个重复10只鸡,试验分两个阶段进行,共56天。采用双因素试验设计并建立炎症应激模型：日粮类型450mg/kg茶皂素)和炎症应激(注射LPS或生理盐水),探讨在日粮中添加茶皂素对黄羽肉鸡在正常生长情况下和炎症应激条件下的生长性能、免疫抗炎功能和抗氧化能力的影响,为开发无害、绿色、安全的动物饲料添加剂提供理论依据。研究结果如下：日粮中添加茶皂素能提高黄羽肉鸡的生长性能,与对照组相比,日采食量有显著提高(P0.05),在一定程度上提高了日增重,降低了料肉比,并能有效缓解LPS刺激对生长带来的负面影响。饲粮中添加茶皂素可在一定程度提高免疫器官指数,添加450mg/kg茶皂素对免疫球蛋白IgA、IgG、IgM和补体C3、C4、IL-2、TNF-α的分泌有明显的促进作用(P0.05)；较高剂量的茶皂素可以在一定程度缓解LPS刺激带来的免疫器官发育缓慢和免疫蛋白分泌抑制,但差异不显著(P0.05),添加300mg/kg茶皂素能有效缓解炎症应激对肉鸡血清抗炎活性造成的负面影响(P0.05)。日粮中添加茶皂素可提高黄羽肉鸡血清和肝脏中抗氧化酶GSH-Px、T-SOD、 T-AOC活性,降低MDA活性,以添加450mg/kg茶皂素对肉鸡抗氧化能力的促进作用最为明显(P0.05)；对0-4周龄黄羽肉鸡,添加450mg/kg茶皂素能有效改善炎症应激对肉鸡血清和肝脏抗氧化能力造成的负面影响(P0.05),对5-8周龄黄羽肉鸡,300mg/kg茶皂素能有效改善炎症应激对肉鸡血清和肝脏抗氧化能力造成的负面影响(P0.05)。LPS可极显著提高黄羽肉鸡法氏囊中炎症信号分子iNOS、NF-κB、Cox-2基因表达水平(P0.01),添加450mg/kg茶皂素剂量的茶皂素可抑制炎症信号分子表达上调(P0.05)。综上所述：茶皂素对黄羽肉鸡具有促生长、提高免疫力、抗炎和抗氧化能力的作用。茶皂素的添加改善了日采食量、日增重和料肉比,促进了免疫器官发育,提高了免疫球蛋白和补体水平和抗氧化酶活性,在炎症应激条件下,茶皂素能有效缓解LPS对肉鸡造成的生长、免疫、抗氧化性能下降,炎性细胞因子过度分泌,信号分子表达上调等负面影响,在机体炎症反应中发挥着重要的缓解作用。
[Abstract]:A total of 480 1 day old yellow feathered broiler chickens, were randomly divided into 8 treatments with 6 replicates of 10 chicks per replicate. The experiment was divided into two stages, a total of 56 days. The two factor experimental design and the establishment of inflammatory stress model: diet type 450mg/kg tea saponin) and inflammatory stress (injection of saline or LPS), to explore in the diets of tea saponin on growth performance of broiler Huang Yu under normal growth conditions and inflammatory conditions, anti-inflammatory effect of immune function and antioxidant ability, for the development of green, harmless, and provide a theoretical basis for the safety of animal feed additives. The results are as follows: in the diet addition of tea saponin can improve the growth performance of broilers, compared with the control group, the daily feed intake was significantly increased (P0.05), to a certain extent, improve the daily gain, lower feed conversion ratio, and can effectively alleviate the stimulation of LPS on the growth of the negative. Ring. The effect of dietary tea saponin can improve the immune organ index in a certain extent, add 450mg/kg of tea saponin on immunoglobulin IgA, IgG, IgM and complement C3, C4, IL-2, TNF- secretion significantly promoted (P0.05); high dose of tea saponin can alleviate LPS to stimulate the development of immune organs slow and immune protein secretion inhibition in a certain degree, but the difference was not significant (P0.05), add 300mg/kg of tea saponin can effectively alleviate the negative impact caused by stress on serum inflammatory and anti-inflammatory activity (P0.05). Dietary supplementation of tea saponin can improve Huang Yu serum and liver antioxidant enzymes GSH-Px, T-SOD, T-AOC activity decreased the activity of MDA, adding 450mg/kg of tea saponin on antioxidative ability of broilers were obvious promoted (P0.05); the 0-4 week old yellow broilers, add 450mg/kg of tea saponin can effectively improve the stress on serum and liver inflammation The negative impact caused by the antioxidant capacity (P0.05), the 5-8 week old yellow broilers, 300mg/kg tea saponin can effectively improve the negative effects caused by inflammatory stress on serum and liver antioxidant ability of broilers (P0.05).LPS could significantly increase the inflammatory signaling in yellow feather broiler bursa in sub iNOS, NF- kappa B, Cox-2 gene the expression level of 450mg/kg (P0.01), add a dose of tea saponin, tea saponin can inhibit the expression of inflammatory signaling molecules (P0.05). Conclusion: tea saponin can promote growth, enhance immunity of Huang Yu broiler, anti-inflammatory and antioxidant capacity for tea saponin added to improve the daily feed intake, daily gain and feed conversion ratio. To promote the development of immune organs, improve the immune globulin and complement levels and antioxidant enzyme activity in inflammatory conditions, tea saponin can effectively alleviate LPS caused by the growth of broiler, immune and antioxidant properties decreased, inflammatory cells The oversecretion of factors, the up-regulation of the expression of signal molecules and other negative effects, play an important role in alleviating the inflammatory response of the body.

【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5

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