20种单味中草药对鸡源多杀性巴氏杆菌的体外抑菌试验

发布时间：2018-02-05 05:43

本文关键词： 鸡多杀性巴氏杆菌中草药体外抑菌试验微量二倍稀释法最小抑菌浓度(MIC)　出处：《黑龙江畜牧兽医》2017年14期 　论文类型：期刊论文

【摘要】：为了研究20种单味中草药对鸡源多杀性巴氏杆菌QHP-1株的体外抑菌效果,试验采用平板琼脂打孔法测定抑菌圈直径大小,改良微量二倍稀释法测定最小抑菌浓度(MIC)。结果表明:鸡源多杀性巴氏杆菌QHP-1株对五味子、黄连、石榴皮、乌梅4种药物极敏,对五倍子、白头翁、黄芩、沙棘、鱼腥草、夏枯草、穿心莲、车前草、黄柏9种药物高敏,对公丁香、苏木、大黄中敏,对薄荷、陈皮、马齿苋、甘草4种药物不敏感。说明五味子、黄连、石榴皮、乌梅4种中草药可以作为防治鸡源多杀性巴氏杆菌复方中草药复方制剂进行进一步的研发。
[Abstract]:In order to study the bacteriostatic effect of 20 kinds of Chinese herbs on QHP-1 strain of Pasteurella multocida in vitro, the diameter of inhibition circle was measured by plate Agar perforation method. The results showed that QHP-1 strain of Pasteurella multocida from chicken was sensitive to Schisandra chinensis, Coptis chinensis, pomegranate peel and black plum. Euphorbia, Radix Scutellariae, Hippophae rhamnoides, Houttuynia cordata, Prunus subtilis, andrographis, Herba plantaris, Phellodendron chinensis, Gao Min, to male clove, sappa, rhubarb Zhongmin, menthol, pericarp, Portulaca oleracea. Four kinds of Chinese herbal medicine of Glycyrrhiza uralensis were insensitive, which indicated that Schisandra chinensis, Coptis chinensis, pomegranate peel and black plum could be further developed as compound Chinese herbal medicine preparation against Pasteurella multocida from chicken.
【作者单位】：河北科技师范学院河北省预防兽医学重点实验室;
【基金】：河北省现代农业产业技术体系蛋鸡产业创新团队岗位项目(HBCT2013090203) 河北省科技支撑项目(12220412D)
【分类号】：S853.74
【正文快照】： 多杀性巴氏杆菌(Pasteurella multocida)属于巴氏杆菌科的巴氏杆菌属,是一种两端钝圆、短杆状或者球杆状的革兰氏阴性菌,根据多杀性巴氏杆菌荚膜抗原和菌体抗原的不同,可以将其分为A、B、D、E、F5个血清型,3个亚种,即多杀亚种、败血亚种及杀禽亚种[1]。禽巴氏杆菌病又称禽霍乱

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