GLP实验动物设施屏障环境消毒方法的研究

发布时间：2018-02-08 19:13

本文关键词： GLP 屏障环境消毒方法　出处：《山东农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本研究选择了山东省食品药品检验所药物安全评价中心在日常消毒工作中经常使用的84消毒液、苯扎溴铵、百毒杀三种消毒剂作为研究对象。三者为含氯类、含季铵盐类具有代表性的消毒液。通过不同浓度的消毒剂在不同作用时间的条件下,使用浸泡、擦拭、喷雾三种消毒措施测定消毒效果。通过试验了解三种消毒剂对GLP实验动物设施屏障环境与饲养用具物品的消毒效果。力求寻找理想的消毒剂和与它匹配的消毒方式。得到试验结果:三种消毒剂在浸泡消毒方法中,均适用于GLP实验动物设施屏障环境中饲养器具(饲养鼠盒、饮水瓶)浸泡消毒。浸泡消毒30min都能达到99.9%以上的灭菌率。有效氯含量为2000mg/l的84消毒液,浓度为0.1%苯扎溴铵,稀释比例为1:100的百毒杀浸泡消毒效果比较好。三种消毒剂浸泡消毒效果与浓度、浸泡作用时间均成正比例关系。三种消毒剂在喷雾消毒方法中,试验结果证明84消毒液、苯扎溴铵、百毒杀均对屏障环境中墙面与环境空气喷雾消毒有一定的消毒和抑菌的效果,其中有效氯含量1000mg/l的84消毒液,浓度为0.05%的苯扎溴铵,稀释比例1:600的百毒杀喷雾消毒效果比较好。三种消毒剂在擦拭消毒方法中,试验结果说明如果每天对GLP屏障环境中墙面与笼架台面擦拭消毒一次,使用有效率含量500mg/l、1000mg/l、2000mg/l的84消毒液;浓度为0.05%、0.1%的苯扎溴铵;稀释比例为1:600、1:200、1:100的百毒杀均可以达到99.9%以上的灭菌率;如果每两天对GLP屏障环境中墙面与笼具表面擦拭一次。使用有效氯含量1000mg/l、2000mg/l的84消毒液;浓度为0.1%苯扎溴铵;稀释比例为1:600、1:200、1:100的百毒杀可以达到99.9%以上灭菌率。采用稀释比例为1:100的百毒杀可以维持99.9%的灭菌率5天。试验中有效氯含量为1000mg/l的84消毒液、浓度为0.1%苯扎溴铵以及稀释比例为1:100的百毒杀擦拭消毒效果最好。上述结果证明三种消毒剂都比较适用GLP实验动物设施屏障环境中消毒工作。浸泡消毒,灭菌效果好的为有效氯浓度2000mg/l的84消毒液、浓度为0.1%苯扎溴铵、稀释比例为1:100的百毒杀。因84消毒液具有腐蚀性,笼具中的笼盖等不锈钢制品消毒建议使用苯扎溴铵与百毒杀浸泡消毒。但从三种消毒剂浸泡消毒效果对比中,84消毒液与百毒杀优于苯扎溴铵消毒剂,更适合浸泡消毒方式。喷雾消毒如选择每日一次时,可选用有效氯含量500mg/l的84消毒液、浓度为0.1%苯扎溴铵、稀释比例为1:600百毒杀可以达到99.9%消毒效果。擦拭消毒三者效果均很可靠,其中百毒杀效果最好。如果每日擦拭一次可选用有效氯含量500mg/l~2000mg/l的84消毒液、浓度为0.05%~0.01%苯扎溴铵、稀释比例为1:600以上的百毒杀。两天擦拭消毒一次,可选用有效氯含量1000mg/l~2000mg/l的84消毒液、浓度为0.1%苯扎溴铵,稀释比例1:600以上的百毒杀。五天擦拭消毒一次可选用1:100的百毒杀。根据比较不同消毒方式下三种消毒剂的消毒试验结果,综合辩证的考虑三种消毒液的安全性、实用性以及性价比等条件。得出这三种消毒剂均比较适用于GLP实验动物设施消毒工作。实际消毒工作中,常使用喷雾消毒与擦拭消毒相结合的方式,其消毒效果比较好。
[Abstract]:This study selected 84 disinfectant safety evaluation center of Shandong province food and drug inspection of drugs often used in daily disinfection work, benzalkonium bromide, three kinds of disinfectant Baidusha as the research object. Three for chlorine, disinfection solution containing quaternary ammonium salt representative. By varying the concentration of the disinfectant in different effect of time under the conditions of use, soak, wipe, spray three kinds of disinfection measures determined the disinfection effect. Understand the three kinds of disinfectants on GLP experimental animal facility barrier environment and feeding utensils disinfection effect by experiment. In order to find the ideal disinfectant and its matching disinfection. Test results have been obtained: three kinds of disinfectants in immersion disinfection in the method, are applicable to the environmental GLP experimental animal facility barrier rearing apparatus (feeding rat box, water bottle) immersion disinfection. Immersion disinfection 30min can achieve sterilization rate of more than 99.9%. Effect of chlorine content of 84 disinfectant solution 2000mg/l, concentration of 0.1% benzalkonium bromide, the dilution ratio of 1:100 Baidusha immersion disinfection effect is better. Three kinds of disinfection disinfectant soaking and concentration, soaking time effect is proportional. Three kinds of disinfectant in disinfection method, the test results show that the 84 disinfectant, benzalkonium bromide, Baidusha havecertain disinfection and antibacterial effect on the environment and the environmental air barrier wall spray disinfection, 84 disinfectant which 1000mg/l effective chlorine content, the concentration of 0.05% benzalkonium bromide, 1:600 dilution poison to kill the disinfection effect of spray is better. Three kinds of disinfectant in disinfection method, test results show that if every day on the wall GLP barrier and cage support table clean disinfection, use efficiency was 500mg/l, 1000mg/l, 2000mg/l 84 disinfectant; the concentration of 0.05%, 0.1% benzalkonium bromide; The dilution ratio of 1:600,1:200,1:100 Baidusha can reach the sterilization rate of more than 99.9%; if every two days on the environment GLP barrier wall and cage surface wipe again. The use of effective chlorine content of 1000mg/l, 2000mg/l concentration of 84 disinfectant; 0.1% benzalkonium bromide; dilution ratio of 1:600,1:200,1:100 Baidusha can reach 99.9% above the sterilization rate. The dilution ratio of 1:100 Baidusha can maintain 99.9% of the sterilization rate of 5 days. The available chlorine content in the experiment is 84 1000mg/l disinfectant, 0.1% concentration of benzalkonium bromide and the dilution ratio of 1:100 Baidusha wipe disinfection effect is best. The results show that three kinds of disinfectants are more suitable environment GLP Experimental Animal Barrier Facilities and disinfection work. Immersion disinfection, sterilization effect of 84 disinfectant effective chlorine concentration of 2000mg/l, concentration of 0.1% benzalkonium bromide, the dilution ratio of 1:100 Baidusha. Because the 84 disinfectant is corrosive, the cage in the cover of stainless steel products such as disinfection suggest the use of benzalkonium bromide and Baidusha immersion disinfection. But from the comparison of three kinds of disinfection disinfectant, disinfectant Baidusha is better than that with 84 benzalkonium bromide disinfectant, more suitable for immersion disinfection. Such as the choice of daily disinfection spray once, 84 disinfectant can choose effective chlorine content of 500mg/l, the concentration of 0.1% benzalkonium bromide, the dilution ratio of 1:600 Baidusha can achieve the disinfection effect of 99.9%. Three disinfection effects are very reliable, which Baidusha best. If a daily wipe 84 disinfectant can choose effective chlorine content of 500mg/l~2000mg/l the concentration of 0.05%~0.01% of benzalkonium bromide, the dilution ratio is more than 1:600 Baidusha. Two days cleaning disinfection, 84 disinfectant can choose effective chlorine content of 1000mg/l~2000mg/l, the concentration of 0.1% benzalkonium bromide, dilution ratio With more than 1:600 Baidusha. Five days a 1:100 can be used to wipe disinfection Baidusha. According to the test results of the three kinds of disinfectant disinfection under different disinfection methods, comprehensive dialectic to consider the safety of three kinds of disinfectant, practical and cost-effective conditions. The three kinds of disinfectant were suitable for sterilization GLP experimental animal facility. The actual work of disinfection, disinfection and disinfection spray is often used in combination, the disinfection effect is good.

【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S851.36

【参考文献】