鸡血藤总黄酮抗炎活性的研究

发布时间：2018-02-09 20:32

本文关键词： 鸡血藤总黄酮抗炎小鼠耳肿胀法炎症因子脂多糖　出处：《黑龙江畜牧兽医》2017年11期 　论文类型：期刊论文

【摘要】：为了探讨鸡血藤总黄酮的抗炎活性,试验采用二甲苯所致小鼠耳肿胀模型和大肠杆菌脂多糖刺激RAW264.7细胞炎症模型,测定鸡血藤总黄酮对小鼠耳肿胀及RAW264.7细胞炎性因子分泌水平的抑制作用。结果表明:体内鸡血藤总黄酮高剂量(100 mg/kg)和中剂量(50 mg/kg)组小鼠耳肿胀率显著低于空白对照组(P0.05),体外鸡血藤总黄酮处理对LPS刺激RAW264.7细胞所致的一氧化氮(NO)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)和白细胞介素6(IL-6)分泌水平的升高具有显著抑制作用(P0.05)。说明鸡血藤总黄酮在体内和体外均具有良好的抗炎效果。
[Abstract]:In order to study the anti-inflammatory activity of total flavonoids of Ampelopsis chinensis, the mouse ear swelling model induced by xylene and the inflammatory model of RAW264.7 cells stimulated by Escherichia coli lipopolysaccharide were used in the experiment. The inhibitory effects of total flavonoids on ear swelling and secretion of RAW264.7 cytokines in mice were determined. The results showed that the ear swelling rate of mice with high dose of flavonoids and 50 mg / kg of middle dose was significantly lower than that of blank group. In the irradiation group P0.05, the in vitro treatment of total flavone of Chicken Rattan had a significant inhibitory effect on the increase of the levels of nitric oxide, TNF- 伪, IL-1 尾 and IL-6) in RAW264.7 cells stimulated by LPS. The results indicated that the total level of TNF- 伪 and IL-6 in RAW264.7 cells was significantly inhibited by the treatment of flavonoids in vitro. The results indicated that the content of TNF- 伪, IL-1 尾 and IL-6IL-6) in RAW264.7 cells stimulated by LPS could be significantly inhibited (P 0.05). Flavonoids have good anti-inflammatory effect in vivo and in vitro.
【作者单位】：广西大学动物科学技术学院;
【基金】：广西自然科学基金项目(2014GXNSFCA118019) 广西教育厅科研项目(KY2015ZD007) “大学生创新创业训练计划”创新训练项目(201610593081)
【分类号】：S853.7

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