菖蒲龙骨散对雏鸡免疫应激的预防作用及机理研究

发布时间：2018-02-10 22:04

本文关键词： LPS 菖蒲龙骨散皮质酮免疫应激雏鸡　出处：《河北农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本试验旨在研究菖蒲龙骨散对LPS致雏鸡免疫应激的预防作用及机理,筛选出最佳组方为临床防治雏鸡免疫应激提供理论依据。将1日龄健康雏鸡180只,随机平均分成6组,每组30只(分3个重复,每个重复10只)。其中,A组为空白对照组,B组为LPS组,C组为LPS+芪参散组,D组为LPS+白术茯苓散组,E组为LPS+菖蒲龙骨散组,F组为LPS+氯丙嗪组。7日龄时,A、B组饲喂基础日粮,C、D、E和F组饲喂添加各组组方的基础日粮,饲喂一星期,在14、16、18、20日龄时,对A组雏鸡腹腔注射生理盐水,每只1 ml;对B、C、D、E和F组雏鸡腹腔注射LPS+生理盐水,每只1 ml,LPS用量为1600μg/kg(BW)。各组分别于14、21、28、35日龄时称重,心脏采取EDTA抗凝血,测定红、白细胞总数,心脏采血离心得血清,测定血液指标,随后剖杀采集胸腺、脾脏、法氏囊,测定其重量,计算免疫器官指数,取法氏囊制作石蜡切片进行HE染色,观察并摄像。试验结果表明,(1)白术茯苓散、菖蒲龙骨散和氯丙嗪能够有效预防免疫应激引起的雏鸡生产性能下降,菖蒲龙骨散的效果更好。(2)白术茯苓散、菖蒲龙骨散和氯丙嗪对免疫应激引起的雏鸡血清总蛋白和白蛋白降低都有一定的预防作用,但相比来说添加菖蒲龙骨散的效果更好。(3)菖蒲龙骨散对免疫应激引起的雏鸡血清IL-1、IL-6升高的预防作用最好。(4)白术茯苓散和菖蒲龙骨散对免疫应激引起的雏鸡血清Ig A、Ig M、Ig G的升高都有预防作用。(5)对免疫应激引起的雏鸡血清皮质酮升高,白术茯苓散和菖蒲龙骨散都有较好的预防效果。结论,菖蒲龙骨散对雏鸡免疫应激的预防作用最好。这为菖蒲龙骨散作为饲料添加剂在养鸡业中的应用提供了理论依据。
[Abstract]:The purpose of this study was to study the preventive effect and mechanism of Acorus Longgu Powder on immune stress induced by LPS in chicks, and to select the best formula to provide theoretical basis for clinical prevention and treatment of immune stress in chicks. 180 healthy chicks of 1 day old were randomly divided into 6 groups. 30 rats in each group (3 repeats, Among them, group A is a blank control group, group B is LPS group, group C is LPS Qi Shen San group, group D is LPS Atractylodes Fuling powder group, group E is LPS Acorus Longgu powder group, group F is LPS chlorpromazine group, group B is fed basic diet at day 7. Group E and F were fed with basal diet supplemented with each group. The chickens in group A were given intraperitoneal injection of normal saline (1 ml / ml) for one week, and the chickens of group A and group F were injected intraperitoneally with LPS. The dosage of LPS was 1 600 渭 g / kg BWN. Each group was weighed at 1421 835 days, and the heart was treated with EDTA anticoagulant. The total number of red and white blood cells were measured, the serum was collected from the heart, and the blood indexes were measured. Then, the thymus, spleen and bursa of Fabricius were collected, their weight was measured, the immune organ index was calculated, and paraffin sections of the bursa of Fabricius were taken to make paraffin sections for HE staining. The experimental results showed that Atractylovir Fuling Powder, Acorus Longgu Powder and chlorpromazine could effectively prevent the performance decline of chicks caused by immune stress, and the effect of Acorus Longgu Powder was better than that of Atractylodes Fuling Powder. Acorus Longgu Powder and chlorpromazine have some preventive effects on the decrease of serum total protein and albumin induced by immune stress. But the effect of Acorus Longgu Powder was better than that of Acorus Longgu Powder.) Acorus Longgu Powder had the best preventive effect on the increase of IL-1 and IL-6 in the serum of chicks induced by immune stress. The best one was Baizhu Fuling Powder and Acorus Longgu Powder on the serum Ig of chicks induced by immune stress. The increase of Ig mg Ig G had a preventive effect on serum corticosterone level in chicks induced by immune stress. Both Atractylodes Atractylodes Fuling Powder and Acorus Longgu Powder have good preventive effects. Conclusion Acorus Longgu Powder has the best preventive effect on immune stress in chicks, which provides a theoretical basis for the application of Acorus Longgu Powder as feed additive in chicken industry.
【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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