血红素铁对妊娠母鼠繁殖成绩及组织铁调基因表达的影响

发布时间：2018-02-11 18:03

本文关键词： 妊娠母鼠血红蛋白铁含量铁调素血红素铁　出处：《动物营养学报》2017年06期 　论文类型：期刊论文

【摘要】：本试验旨在探索血红素铁与硫酸亚铁(FeSO_4)对妊娠母鼠繁殖成绩,妊娠母鼠组织与胎鼠铁含量,妊娠母鼠组织铁调素(hepcidin)、膜铁转运蛋白(Fpn)、猫白血病病毒C亚类受体(Flvcr)、转铁蛋白受体1(Tfr1)、转铁蛋白受体2(Tfr2)、二价金属转运体1(DMT1)和血红素转运蛋白(HCP)表达的影响。随机选取2月龄体况接近的昆明小鼠母鼠80只,随机分为8组,分别为对照组、缺铁组、血红素铁组(15、60、90 mg/kg血红素铁)、FeSO_4组(75、300、450 mg/kg FeSO_4),每组10只。配种受孕起对照组饲喂正常饲粮(基础饲粮中添加400 mg/kg FeSO_4);其他各组均饲喂基础饲粮,妊娠第10~13天注射40 mg/kg去铁胺(DFO),诱导妊娠母鼠缺铁模型;妊娠第14天血红素铁组和FeSO_4组开始在基础饲粮中添加血红素铁或FeSO_4,缺铁组不添加。试验期为妊娠后1~20 d。结果表明:1)60 mg/kg血红素铁组胎鼠重最高,极显著高于对照组与缺铁组(P0.01)。2)60 mg/kg血红素铁组与450 mg/kg FeSO_4组母鼠血液血红蛋白(HGB)含量、红细胞数(RBC)和红细胞容积(HCT)极显著高于缺铁组(P0.01)。3)60 mg/kg血红素铁组胎鼠铁含量最高,极显著高于对照组与FeSO_4组(P0.01);450 mg/kg FeSO_4组母鼠肝脏、脾脏和胎盘铁含量均为最高。4)90 mg/kg血红素铁组和450 mg/kg FeSO_4组母鼠肝脏hepcidin表达量较高,极显著高于缺铁组与对照组(P0.01);15 mg/kg血红素铁组母鼠肝脏Fpn、Tfr2表达量较高,极显著高于对照组和缺铁组(P0.01);60 mg/kg血红素铁组母鼠肝脏Tfr1、Flvcr表达量较高,极显著高于对照组和缺铁组(P0.01);75 mg/kg FeSO_4组母鼠肝脏Tfr1表达量较高,极显著高于对照组和缺铁组(P0.01)。5)缺铁组母鼠十二指肠Fpn、HCP、DMT1、Flvcr表达量均极显著高于对照组(P0.01)。6)缺铁组母鼠胎盘Fpn、Tfr1、DMT1、Flvcr、HCP表达量极显著高于对照组(P0.01);90 mg/kg血红素铁组和450 mg/kg FeSO_4组母鼠胎盘hepcidin表达量较高,极显著高于缺铁组与对照组(P0.01)。7)饲粮血红素铁添加量为61.00 mg/kg或FeSO_4添加量为336.11 mg/kg时,胎鼠铁含量最高;饲粮血红素铁添加量为93.49 mg/kg时,母鼠肝脏铁含量最高。综合得出,母鼠饲粮中添加适宜量的血红素铁或FeSO_4均可显著促进胎鼠增重,诱导母鼠靶组织铁调基因的表达,提高母鼠组织和妊娠20 d胎鼠机体铁含量;HCP和Flvcr在母鼠肠道对血红素铁吸收或胎盘转运起至关重要的作用,但肠道吸收或胎盘转运FeSO_4主要以DMT1和Tfr2为主。关键词:妊娠母鼠;血红蛋白;铁含量;铁调素;血红素铁
[Abstract]:The aim of this study was to investigate the reproductive performance of pregnant rats, the tissue of pregnant rats and the content of iron in fetal mice by heme iron and FeSO4. The effects of ferritin hepcidinine, membrane iron transport protein (FpnnP), feline leukemia virus subclass C receptor (Flvcrn), transferrin receptor 1 (Tfr1), transferrin receptor 2 (Tfr2), divalent metal transporter 1 (DMT1) and heme transporter (HCP) were investigated. 80 female Kunming mice with similar body condition at 2 months of age, They were randomly divided into 8 groups: control group, iron deficiency group and heme iron group for 90 mg/kg heme iron and FeSO4 + 4 groups with 45 mg/kg FeSO4 and 10 rats in each group. The control group was fed with normal diet (400 mg/kg FeSO4 in basic diet), and all other groups were fed basic diet, and all other groups were fed with basic diet, and the other groups were fed with basic diet, and the control group was fed with normal diet (400 mg/kg FeSO4 +), and all the other groups were fed with basic diet, and all the other groups were fed with basic diet. On the 13th day of gestation, 40 mg/kg deferramide was injected to induce the model of iron deficiency in pregnant rats. On the 14th day of pregnancy, heme iron group and FeSO_4 group began to add heme iron or FeSO4 in basic diet, but iron deficiency group did not add heme iron. The contents of hemoglobin (HGB), erythrocyte count (RBC) and erythrocyte volume (RBC) in the heme iron group and the 450 mg/kg FeSO_4 group were significantly higher than those in the iron deficiency group and the iron deficiency group, and the highest iron content was found in the fetal rats in the iron deficiency group. The expression of hepcidin in liver, spleen and placenta was significantly higher than that in the control group and FeSO_4 group, and the expression of hepcidin was higher in the liver, spleen and placenta of the female rats than those in the control group and the FeSO_4 group. The expression of hepcidin in the liver of the female rats was significantly higher than that of the control group and the FeSO_4 group. It was significantly higher than that of iron deficiency group and control group for 15 mg/kg heme iron group. The expression of Fpnntf2 in liver of female rats was significantly higher than that of control group and iron deficiency group. The expression of Tfr1 flvcr in liver of female rats was significantly higher than that of control group and iron deficiency group for 60 mg/kg heme iron group, and the expression of Tfr1 flvcr in liver of female rats was significantly higher than that of control group and iron deficiency group for 60 mg/kg. The expression of Tfr1 in liver of female rats was significantly higher than that of control group and iron deficiency group. The expression of Tfr1 in liver of female rats was higher than that of control group and iron deficiency group. The expression of Fpnntrol / DMT1 / Flvcr in the duodenum of the iron-deficient group was significantly higher than that of the control group (P0.01 / 6).) the expression of Fpnnntrol Tfr1DMT1 / Flvcr-HCP in placenta of the iron-deficient group was significantly higher than that of the control group (P0.01 / 90 mg/kg heme iron group and 450 mg/kg FeSO_4 group, respectively.) the expression of Fpnntropium in the placental placenta of the iron-deficient group was significantly higher than that in the control group (P 0.01) and the female fetus in the 450 mg/kg FeSO_4 group was significantly higher than that in the control group. The expression of hepcidin was high. The iron content of fetal rats was the highest when dietary heme iron addition was 61.00 mg/kg or FeSO_4 addition was 336.11 mg/kg, and the highest iron content in maternal liver was obtained when dietary heme iron supplementation was 93.49 mg/kg, respectively, which was significantly higher than that in iron deficiency group and control group (P 0.01g 路7), and the content of heme iron in maternal liver was the highest when the content of heme iron was 61.00 mg/kg or 336.11 mg/kg. The addition of appropriate amount of heme iron or FeSO_4 in maternal diet could significantly promote the weight gain of fetal mice and induce the expression of iron regulatory gene in the target tissues of maternal mice. To increase the iron content in maternal tissues and fetal mice on the 20th day of gestation, HCP and Flvcr play an important role in the absorption of heme iron or placental transport in maternal intestines, but DMT1 and Tfr2 are the main components in intestinal absorption or placental transport of FeSO_4. Hemoglobin; iron content; iron modulin; heme iron
【作者单位】：云南农业大学动物科学技术学院云南省动物营养与饲料重点试验室;
【基金】：云南省重大科技计划——生物育种(2012ZA018-3)
【分类号】：S816

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