魏氏梭菌与猪瘟病毒混合感染的诊断

发布时间：2018-02-17 06:04

本文关键词： 断奶仔猪魏氏梭菌(C.perfringens) 猪瘟病毒混合感染诊断　出处：《黑龙江畜牧兽医》2017年14期 　论文类型：期刊论文

【摘要】：2016年4月份天津市某小型养殖场14头35日龄左右的断奶仔猪发病,临床表现为体温升高,站立不稳,腹胀、腹泻,有的无任何征兆而突然死亡。为了对该病例进行确诊,在病理剖检过程中采集了发病猪病变明显的组织器官,结合临床病理变化,通过细菌分离及生化鉴定、RT-PCR检测等方法对病原进行鉴别诊断。病理组织学观察结果显示胃肌层和黏膜下层出血,淋巴结周围组织中有大量红细胞,脾脏呈干酪样坏死;镜检可见粗大的阳性杆菌;生化试验结果与魏氏梭菌特性一致;猪瘟病毒E2基因特异性引物扩增结果为阳性,伪狂犬病毒、猪繁殖与呼吸综合征病毒、传染性胃肠炎病毒、轮状病毒和流行性腹泻病毒的扩增结果均为阴性。根据实验室检测结果并结合临床症状综合分析,最终确诊该病例为魏氏梭菌与猪瘟病毒的混合感染。
[Abstract]:In April 2016, 14 weaned piglets of about 35 days old in a small farm in Tianjin fell ill with elevated body temperature, unstable standing, abdominal distension, diarrhea and sudden death without any sign. In the process of pathological dissection, the tissues and organs of the diseased pigs were collected and combined with the clinicopathological changes. The results of histopathological observation showed that the gastric myometrium and submucous layer bleeding, there were a lot of red blood cells in the surrounding lymph nodes, and the spleen was caseous necrosis. The results of biochemical tests were consistent with the characteristics of Clostridium Weissi. The results of specific primer amplification of E2 gene of CSFV were positive, pseudorabies virus, porcine reproductive and respiratory syndrome virus, infectious gastroenteritis virus, The results of amplification of rotavirus and epidemic diarrhea virus were negative. According to the laboratory test results and clinical symptom analysis, the case was confirmed as a mixed infection of Clostridium Wei and classical swine fever virus (CSFV).
【作者单位】：天津农学院动物科学与动物医学学院;
【基金】：国家自然科学基金项目(31572492;31072109) 国家级大学生创新创业训练计划项目(201510061005) 天津市自然科学基金重点项目(12JCZDJC22100) 天津市农委农业科技示范推广项目(201202110) 天津市兽医生物技术优秀科研创新团队项目(TD12-5019) 天津市兽医学重点学科“领军人才培育计划”人选项目天津市优秀科技特派员项目(2016078)
【分类号】：S858.28

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