茶多酚作为驱虫保健剂对羔羊生长的影响

发布时间：2018-02-20 23:33

本文关键词： 茶多酚羔羊生产性能 FEC值肉品质　出处：《吉林农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本试验目的是:在感染捻转血矛线虫羔羊的日粮中添加不同剂量的茶多酚(Gree Tea Polyphenols,GTP),观测GTP对捻转血毛线虫是否具有驱虫作用,为生产实践提供理论依据。选取30头平均日龄在120±10.3 d,体重为24.8±2.4 kg的雄性乌珠穆沁羊,随机平均分为5组,采用完全随机化法进行为期56d的饲养试验。试验处理组分别为:(1)未感染捻转血矛线虫,未饲喂GTP处理组(UNINF组);(2)感染捻转血矛线虫,日粮未添加GTP组(INFGTP0组);(3)感染捻转血矛线虫,日粮中GPT添加量为2 g/kg组(INFGTP2组);(4)感染捻转血矛线虫,日粮中GPT添加量为4 g/kg组(INFGTP4组);(5)感染捻转血矛线虫,日粮中GPT添加量为6 g/kg组(INFGTP6组)。GTP添加剂量以干物质为基础进行计算。饲养试验期间,选取0d、14d、28d、42d、56d的清晨,进行称重、采集血液、从直肠处收集粪便样品。制备血浆,用于测定PCV值、血液生化指标和血液氨基酸含量;使用McMaster法测定FEC值。在饲养试验的51~55d进行全收粪法的消化试验。采集粪便样品用于概略养分分析。在饲养试验结束后,屠宰所有试验羊,取出皱胃和左侧背最长肌。使用显微镜检查每头羊的皱胃内捻转血矛线虫成虫数量、性别。测定肉品质指标和肉中氨基酸含量。试验结果表明,感染捻转血毛线虫会降低羔羊的平均日增重,增加饲料转化率(P0.001),但对干物质采食量和各个营养物质的消化率无影响。日粮添加GTP组的FEC值高于未感染组,但是低于INFGTP0组。感染捻转血毛线虫的羔羊的PCV值低于未感染捻转血毛线虫的羔羊,但PCV值随着GTP的添加量的增加而上升。在感染捻转血矛线虫的羊的日粮中添加不同水平的GTP可以显著减少捻转血矛线虫总数、雄虫数和雌虫数(P0.0001)。在饲养试验末期,INFGTP6组的捻转血矛线虫总数、雄虫数和雌虫数降低至未感染水平(P0.05)。总蛋白浓度最高值出现在56d时INFGTP6组,最低值则为28d时INFGTP2组。INFGTP0组的羔羊,其肉色比较暗沉,而日粮添加GTP组的羔羊肉色则比较鲜红。综上所述,日粮添加4~6g/kg的茶多酚可以通过降低羔羊体内寄生虫数量,来增强羔羊对捻转血毛线虫的抵抗能力,缓解捻转血毛线虫感染导致的不利影响。
[Abstract]:The aim of this study was to investigate the effect of different doses of tea polyphenols Tea polyphenolsm (GTPN) on the treatment of hemocaterpillar twirls by adding different doses of tea polyphenols to the diet of the lamb infected with Hematodes twirlosa, and to observe whether GTP has the effect of insect repellent on the twisting blood caterpillar. To provide theoretical basis for production practice, 30 male Wuzhimuqin sheep with an average age of 120 卤10.3 days and a body weight of 24.8 卤2.4 kg were randomly divided into 5 groups. A 56 day feeding trial was carried out with complete randomization method. The experimental group was treated with 1 / 1) without infection of blood nematode, without GTP treatment group (UNINF group) infected with blood spear nematode, and diet group without GTP group (INFGTP0 group) infected with blood spear nematode. 2 g / kg GPT was added to 2 g / kg group and 4 g / kg of GPT was added to the diet, and 4 g / kg of GPT was added to 4 g / kg group, and 4 g / kg of GPT was added to 4 g / kg group. The dosage of GPT additive was calculated on the basis of dry matter. During the feeding experiment, during the feeding experiment, the rats were selected for weighing, collecting blood, collecting fecal samples from rectum and measuring PCV value during feeding experiment. Biochemical indexes of blood and content of amino acid in blood; FEC value was measured by McMaster method. Digesting test of total fecal collection was carried out at 51g / 55d of feeding test. Fecal samples were collected for general nutrient analysis. After feeding trial, all the sheep were slaughtered. Take out the abomasum and the left longissimus dorsi muscle. Use the microscope to examine the number, sex, meat quality index and amino acid content of the blood spear nematode in the abomasum of each sheep. The average daily gain of lambs was decreased and feed conversion rate was increased P0.001, but it had no effect on dry matter intake and digestibility of various nutrients. The FEC value of diet supplemented with GTP group was higher than that of uninfected group. But lower than that of INFGTP0 group. The PCV value of lambs infected with Haematoderma was lower than that of lambs without infection. However, the PCV value increased with the increase of GTP. Adding different levels of GTP to the diets of sheep infected with Haemondosis significantly reduced the total number of blood nematode twists. At the end of the feeding experiment, the total number of blood spear nematode, the number of male and female decreased to the uninfected level (P 0.05). The highest concentration of total protein appeared at 56 d in INFGTP6 group, and the lowest value was 28 d in INFGTP2 group .INFGTP0 group. The meat color of lambs was darker than that of lambs fed with GTP. To sum up, the diet supplemented with 4g / kg tea polyphenols could increase the lambs' resistance to twirling caterpillars by reducing the number of parasites in the lambs. To alleviate the adverse effects caused by the infection of Haematoderma twirlis.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.26

【参考文献】