ALV-J不同接种途径诱导SPF鸡免疫抑制状况的比较分析

发布时间：2018-02-21 10:50

本文关键词： J亚群禽白血病病毒(ALV-J) 人工感染 SPF鸡水平传播免疫抑制　出处：《中国兽医学报》2017年11期 　论文类型：期刊论文

【摘要】：将1日龄SPF鸡分别通过口服、点眼、腹腔注射、肌肉注射的方式感染J亚群禽白血病病毒(subgroup J avian leucosis virus,ALV-J),监测各感染组鸡的生长性能、带毒和排毒规律,并对其病毒血症、相关免疫学指标及抗体反应进行动态检测。结果显示:各感染组鸡的生长性能和免疫应答水平存在明显差异。从感染后第2周开始腹腔注射和肌肉注射组可检出泄殖腔排毒和病毒血症,此后持续带毒、排毒;体质量、免疫器官指数以及淋巴细胞转化率、CD4+和CD8+T淋巴细胞数、细胞因子(IL-2、IL-4和IFN-γ)的分泌量显著低于对照组;抗体转阳不明显。而口服、点眼感染组与对照组相比引起的免疫抑制程度并不显著。结果表明:1日龄雏鸡感染ALV-J容易造成免疫抑制,其中腹腔注射和肌肉注射感染造成鸡体带毒、排毒和免疫抑制的能力最强。本试验明确了鸡群ALV-J水平传播的不同途径与鸡的感染力、免疫抑制力之间的关系,为研究ALV-J的感染机制、种群净化以及防控提供了理论依据。
[Abstract]:The 1-day-old SPF chickens were infected with subgroup J avian leucosis virus of subgroup J avian leucosis virus by oral administration, intraperitoneal injection, and intraperitoneal injection. The growth performance, virulence and detoxification of each infected group were monitored. The results showed that there were significant differences in growth performance and immune response level among the infected chickens. From the second week after infection, the chickens could be detected by intraperitoneal injection and intramuscular injection. Cloacal detoxification and viremia, After that, the levels of persistent viral infection, detoxification, body mass, immune organ index, lymphocyte transformation rate, CD4 and CD8 T lymphocyte count, cytokines IL-2, IL-4 and IFN- 纬) were significantly lower than those in the control group. The results showed that ALV-J infection of 1 day old chicks was easy to cause immunosuppression, in which intraperitoneal injection and intramuscular injection resulted in virus infection in chicken body, and the immune inhibition was not significant in the group of point eye infection compared with the control group, the results showed that the infection of 1 day old chicks could easily cause immunosuppression. The ability of detoxification and immunosuppression was the strongest. This experiment clarified the relationship between the different routes of chicken ALV-J level transmission and chicken's infectivity and immunosuppressive ability, which provided the theoretical basis for studying the infection mechanism, population purification and prevention and control of ALV-J.
【作者单位】：山东农业大学动物科技学院/山东省动物生物技术与疫病防治重点实验室/山东省畜禽疫病防治工程技术研究中心;
【基金】：山东省高校中医药抗病毒协同创新中心项目(XTCX2014B01-08) 山东科技发展计划资助项目(2012GNC11020) 山东省“双一流”奖补资金项目(SYL2017YSTD11)
【分类号】：S858.31

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