我国羊巴贝斯虫trap基因结构及遗传进化分析

发布时间：2018-02-22 23:45

本文关键词： 羊巴贝斯虫 trap基因基因结构系统发生树　出处：《畜牧兽医学报》2017年07期 　论文类型：期刊论文

【摘要】：本研究拟解析我国羊巴贝斯虫trap基因的结构特征,并以其为分子标志开展遗传进化分析,为理清我国羊巴贝斯虫的分类关系提供数据。用公布的巴贝斯虫trap基因序列BLAST两个羊巴贝斯虫全基因组本地数据库,以获得的保守序列片段为模板,设计PCR引物;克隆我国分离的6株羊巴贝斯虫trap基因全长序列,分析其序列结构特征;比对序列相似性,构建系统发生树,分析其遗传进化关系。结果克隆获得6株羊巴贝斯虫trap基因,分析结果显示,其具有3、4、5个内含子的三种内含子数目,预测的开放阅读框(ORF)大小分别为1 944、2 100/2 142和2 286bp;氨基酸序列分析显示,其都具有TRAP蛋白家族特征性的vWFA、TSR、跨膜域和CTD结构域;进化关系分析显示,感染我国羊的巴贝斯虫被分成3组,组内序列相似性为99.8%~100%,而组间为43.6%~74.6%。系统发生树上6株羊巴贝斯虫被分到两大枝,羊巴贝斯虫未定种和莫氏巴贝斯虫,而且莫氏巴贝斯虫又被分成两小枝。6株羊巴贝斯虫trap基因结构存在差异,但其蛋白特征性的结构域较为保守;我国分离的羊巴贝斯虫为两个种,但莫氏巴贝斯虫中可能存在两个亚种。
[Abstract]:The aim of this study was to analyze the structural characteristics of the trap gene of Babes's worm in China and to use it as a molecular marker to carry out genetic and evolutionary analysis. To provide data for sorting out the taxonomic relationship of Babes's sheep in China, PCR primers were designed using the published BLAST gene sequence of Babes's trap gene and the local database of the whole genome of Babes's sheep, using the conserved sequence fragments as template. The full-length sequence of trap gene of six sheep Babes worm isolated from China was cloned, and its structural characteristics were analyzed. The sequence similarity was compared, the phylogenetic tree was constructed, and the genetic evolution relationship was analyzed. Results six trap genes of Babes were cloned. The results showed that there were 3 introns with 3 introns and 5 introns, and the predicted ORFs of open reading frames were 1 944C 2 100/2 142 and 2 286 BP, respectively, and the amino acid sequence analysis showed that, The vWFA-TSRs, transmembrane domain and CTD domain were all characteristic of TRAP protein family, and the evolutionary relationship analysis showed that the Babes parasites infected with Chinese sheep were divided into three groups. The similarity of the sequence in the group was 99.8% and 43.6% and 74.6%, respectively. Six sheep Babes insects were divided into two branches, Babes and Babes Mori. Moreover, the trap gene structure of Babes Mori was divided into two branchlets, but the characteristic domain of its protein was conserved, and there were two species of Babes isolated in China. But there may be two subspecies in Babes Mori.
【作者单位】：中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室;
【基金】：国家重点基础研究发展计划(973)项目(2015CB150300) 国家自然科学基金(31072130) 农业科技创新工程(ASTIP)
【分类号】：S852.723

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