饲粮中胆碱添加水平对3～5月龄獭兔肝脏脂肪代谢的影响

发布时间：2018-02-23 19:48

本文关键词： 胆碱獭兔肝脏脂肪代谢　出处：《动物营养学报》2017年07期 　论文类型：期刊论文

【摘要】：本试验旨在研究饲粮中胆碱添加水平对3~5月龄獭兔肝脏脂肪代谢的影响。选用200只体重相近的3月龄獭兔,随机分成5组(每组40个重复,每个重复1只),分别饲喂在基础饲粮基础上添加0(对照)、200、400、600和800 mg/kg的50%氯化胆碱(折算为胆碱的添加水平后分别为0、87、174、261和348 mg/kg)的试验饲粮,试验饲粮实测胆碱水平分别为812、902、993、1 086和1 170 mg/kg。预试期7 d,正试期53 d。结果表明:饲粮中胆碱添加水平显著影响肝脏中单不饱和脂肪酸和多不饱和脂肪酸的含量(P0.05)。与对照组相比,饲粮中添加87、174、261和348 mg/kg的胆碱显著增加血清中类胰岛素样生长因子1(IGF-1)和瘦素(LEP)的含量(P0.05),饲粮中添加174和261 mg/kg的胆碱显著增加血清中生长激素(GH)的含量(P0.05),饲粮中添加261和348 mg/kg的胆碱显著增加血清中脂蛋白酯酶(LPL)的活性和极低密度脂蛋白(VLDL)的含量(P0.05),饲粮中添加261 mg/kg的胆碱显著提高肝脏中肉碱棕榈酸转移酶1(CPT1)m RNA的表达水平(P0.05)。综上所述,饲粮中添加261 mg/kg的胆碱(饲粮实测胆碱水平为1 086 mg/kg)改变了獭兔肝脏中脂肪酸组成,促进了肝脏中脂肪酸氧化关键基因CPT1的表达,同时加强了肝脏中甘油三酯(TG)的输出过程。
[Abstract]:The aim of this study was to study the effect of dietary choline supplementation on liver fat metabolism in 3 ~ 5 month old Rex rabbits. 200 3 month old rex rabbits of similar body weight were randomly divided into 5 groups (40 replicates in each group). Each repeater was fed with a diet of 50% choline chloride (converted to a choline addition level of 0 77174261 and 348 mg / kg, respectively) added to the basal diet (control group 200g, 400m, 600 and 800 mg/kg, respectively). The measured levels of choline in the diet were 812902 / 993 / 1086 and 1 170 mg / kg / kg, respectively. The results showed that dietary choline supplementation significantly affected the contents of monounsaturated fatty acids and polyunsaturated fatty acids in the liver after 7 days of pretest and 53 days of positive test. Compared with the control group, the levels of choline in the diet significantly affected the contents of monounsaturated fatty acids and polyunsaturated fatty acids in the liver. Dietary choline supplementation of 87,174,261 and 348 mg/kg significantly increased the levels of insulin-like growth factor-1 (IGF-1) and leptin (leptin) in serum (P 0.05), the addition of choline of 174 and 261 mg/kg to diet significantly increased serum growth hormone (GH) content (P0.05), and dietary supplementation of choline to diet increased serum growth hormone (GH) content. Choline of 261 and 348 mg/kg significantly increased the activity of lipoprotein esterase (LPL) and the content of very low density lipoprotein (VLDL) in serum, and the level of carnitine palmitate transferase 1 (CPT1) RNA expression in liver was significantly increased with the addition of choline of 261 mg/kg in diet. Dietary choline of 261 mg/kg (dietary measured choline level of 1 086 mg / kg) changed fatty acid composition in liver of Rex rabbits, promoted the expression of key gene CPT1 in liver fatty acid oxidation, and enhanced the output process of triglyceride in liver.
【作者单位】：山东农业大学动物科技学院;
【基金】：现代农业产业技术体系建设专项(CARS-44-B-1) 山东农业大学青年科技创新基金项目(2015—2016);山东农业大学博士后科学基金项目(2015—2017) 中国博士后科学基金项目(2015M58061)
【分类号】：S829.1

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