绵羊Lpin3基因多态性及其与尾型和屠宰性状的关联研究

发布时间：2018-02-23 20:04

本文关键词： 绵羊 Lpin基因单核苷酸多态性关联研究　出处：《中国畜牧杂志》2017年07期 　论文类型：期刊论文

【摘要】：为探究Lpin3基因变异对绵羊肉用性状的影响,选择广灵大尾羊和小尾寒羊两品种脂尾型绵羊为研究对象,采用DNA直接测序法检测Lpin3基因5'非编码区部分序列的单核苷酸多态性(SNPs),并分析SNPs与尾型和屠宰性状的关联。结果检测到Lpin3基因5'非编码区起始密码子上游约1 200 bp的DNA序列13个SNPs,其中-334~-332ins TAA显著降低了小尾寒羊的尾宽(P0.05),提高了小尾寒羊的屠宰性状(P0.05),在小尾寒羊中的突变频率显著高于广灵大尾羊(P0.05),对广灵大尾羊各性状无显著影响(P0.05)。尽管不显著,小尾寒羊中-443 TC与-334~-332 ins TAA具有相似的遗传效应。该研究可为绵羊Lpin3基因功能及肉质性状分子标记辅助选择提供依据。
[Abstract]:In order to investigate the effect of Lpin3 gene variation on meat traits of sheep, two types of fat-tailed sheep, Guangling large tail sheep and small tail Han sheep, were selected as the research objects. The single nucleotide polymorphisms (SNPs) of 5'non-coding region partial sequence of Lpin3 gene were detected by DNA direct sequencing, and the association of SNPs with tail type and slaughter traits was analyzed. The results showed that the 5'noncoding region of Lpin3 gene was about 1,200 upstream of the start codon of 5'non-coding region. The BP DNA sequence was 13 SNPs, of which -334N -332ins TAA significantly reduced the tail width of small tail Han sheep (P0.05N), and increased the slaughter trait P0.05N of small tail Han sheep. The mutation frequency in small tail Han sheep was significantly higher than that in small tail Han sheep (P0.05A), but there was no significant difference among the traits of Guangling large tail sheep. P0.05, although not significant, The genetic effects of -443TC and -334n-332 ins TAA were similar in small tail Han sheep. This study could provide evidence for molecular marker assisted selection of Lpin3 gene function and meat quality traits in sheep.
【作者单位】：山西农业大学动物科技学院;天津农学院动物科学与动物医学学院;
【基金】：国家自然科学基金(31372292) 天津农学院科技发展基金(2013N01) 天津市高校创新团队建设项目(TD12-5019)
【分类号】：S826.2

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