小花棘豆中毒对家兔睾丸生精细胞凋亡的影响

发布时间：2018-02-23 20:27

本文关键词： 小花棘豆苦马豆素生精细胞细胞凋亡细胞周期　出处：《西南农业学报》2017年01期 　论文类型：期刊论文

【摘要】：研究小花棘豆中毒对家兔睾丸生精细胞凋亡的影响,进一步探讨小花棘豆的中毒机理。将24只雄性家兔随机分为对照组和试验Ⅰ、Ⅱ、Ⅲ组,试验组分别按照Ⅰ组15%(苦马豆素含量30 mg/kg)、Ⅱ组30%(苦马豆素含量60 mg/kg)、Ⅲ组45%(苦马豆素含量90 mg/kg)的比例添加小花棘豆,对照组仅饲喂苜蓿干草,试验期70 d。分别于攻毒后第14、35、70天每次每组随机采集2只家兔的睾丸,通过TUNEL法定位,Hoechst 33258荧光染色细胞核,流式细胞仪测定细胞凋亡率和细胞周期。从第35天开始,试验组家兔生精细胞凋亡率均极显著升高(P㩳0.01),表现为细胞膜下陷,细胞器浓缩,细胞核碎裂等,并出现凋亡小体。试验组家兔睾丸单倍体细胞和二倍体细胞凋亡率均极显著升高(P㩳0.01),但四倍体细胞数量无显著变化。与对照组相比,中毒家兔睾丸生精细胞G0/G1期数量升高,S期细胞数量降低,而且G1期与S期细胞数量呈显著的负相关。小花棘豆中毒可导致家兔睾丸组织生精细胞凋亡,且与小花棘豆毒性成分呈现一定的时间-剂量效应。
[Abstract]:Study on the effects of Oxytropis glabra DC poisoning of rabbit testis spermatogenic cell apoptosis and further explore the mechanism of poisoning of Oxytropis glabra. 24 male rabbits were randomly divided into control group and experimental group I, II, III, respectively according to the experimental group 1 was 15% (swainsonine in 30 mg/kg), group II (30% swainsonine the content of 60 mg/kg), group 45% (swainsonine content 90 mg/kg) added o.glabra, the control group only fed alfalfa hay, a trial period of 70 D. respectively in 14,35,70 days after inoculation of each group were randomly collected 2 rabbits testis by TUNEL method, Hoechst 33258 fluorescence staining, apoptosis rate and cell cycle were determined by flow cytometry. From the thirty-fifth day, the experimental group in the apoptosis rate of spermatogenic cells increased significantly (P? 0.01), showed the cell membrane subsidence, organelle concentration, nuclear fragmentation, and apoptotic bodies. The experimental group of rabbit testis The apoptosis rate of haploid cells and pill diploid cells increased significantly (P? 0.01), but the number of tetraploid cells. No significant changes compared with the control group, the number of poisoning of rabbit spermatogenic cells in G0/G1 phase increased, the number of cells in S phase decreased, and had significant negative correlation between the number of G1 phase and S phase cells. Flowers Oxytropis poisoning can cause rabbit testis spermatogenic cell apoptosis, and presented time and dose effect of toxic components of Oxytropis glabra.

【作者单位】：塔里木大学动物科学学院;新疆生产建设兵团塔里木畜牧科技重点实验室;
【基金】：国家自然科学基金(31460678) 国家星火计划项目(2015GA891015) 兵团塔里木畜牧科技重点实验室开放课题(HS201409)
【分类号】：S859.8

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