用于兽药掺假检测的拉曼光谱仪光学系统设计

发布时间：2018-02-24 03:25

本文关键词： 拉曼光谱兽药检测光学设计透射式系统　出处：《中国科学院研究生院(长春光学精密机械与物理研究所)》2015年硕士论文　论文类型：学位论文

【摘要】：兽药可以有效防治动物的疾病,对畜牧业的健康发展发挥着重要的作用。然而,一些企业在药品中非法掺杂其他物质冒充药物的有效成分,从而延误治疗,给养殖户造成了巨大的损失。这些掺杂成分与药品性状相似,肉眼难以分辨,需要专业的仪器进行检测。分析检测技术需要满足准确、快速、简便、经济等要求,其中任何一项的缺失,都会限制该技术的推广使用。目前,假药检测方法主要有化学法、色谱法和光谱法。其中,拉曼光谱法是以拉曼散射为基础的光谱分析技术,它具有速度快、非接触、无损伤、所需样品量少且无需预处理等优点,非常适合药品的现场快速检测。随着便携式拉曼光谱仪的出现和化学计量学方法的发展,拉曼光谱分析在药品检测方面的优势更加凸显。本文通过实验进行了兽药掺假的拉曼光谱检测,并设计了用于兽药掺假检测的拉曼光谱仪光学系统,主要内容包括:(1)本文通过拉曼光谱实验与化学计量学方法相结合,研究了兽药掺假的拉曼光谱分析方法,为拉曼光谱仪的设计提供实验指导。首先介绍了偏最小二乘法的定性判别和定量分析原理,然后以兽药中的常用药青霉素作为研究对象,基于偏最小二乘法,对掺葡萄糖和淀粉的青霉素样品进行了定性判别,并对掺假药品中的青霉素含量进行了定量分析。其中包括校正集的选取,奇异样本剔除以及各种预处理方法及其组合对模型的影响等。(2)本文根据兽药检测的需求,设计了便携式拉曼光谱仪的外光路系统。外光路系统包括激光激发和散射光收集两部分。激发光路与激光器输出光纤的数值孔径相匹配,放大率为1:1,系统的点列图大小与爱里斑相当,聚焦能力强。散射光收集光路的物方数值孔径达到0.44,能够使更大角度的拉曼散射光进入系统。所设计的散射光收集光路,零视场和50μm视场的点列图的均方根半径分别为3.949μm和5.074μm,半径10μm的圆内能量超过95%。(3)本文设计了透射式体全息光栅分光的拉曼光谱仪光学系统。根据Kogelnik耦合波理论,分析了体全息光栅的衍射特性;设计了透射式拉曼光谱仪光学系统,该系统的长度小于10cm,物方数值孔径达到0.22,在790-1014nm的光谱范围内,分辨率为0.6nm。
[Abstract]:Veterinary drugs can effectively prevent and cure animal diseases and play an important role in the healthy development of animal husbandry. However, some enterprises illegally adulterate other substances into drugs and impersonate the active components of drugs, thus delaying treatment. These adulterated ingredients are similar to the properties of drugs, difficult to distinguish with the naked eye, and need professional instruments to detect. The analytical and detection techniques need to meet the requirements of accuracy, speed, simplicity, economy, etc. The lack of any of them will limit the use of the technology. At present, the methods for detecting counterfeit drugs are mainly chemical, chromatographic and spectroscopic methods, among which Raman spectroscopy is a spectroscopic technique based on Raman scattering. It has the advantages of high speed, no contact, no damage, less sample quantity and no pretreatment, etc. It is very suitable for the field rapid detection of drugs. With the development of portable Raman spectrometer and chemometrics, The advantage of Raman spectrum analysis in drug detection is more prominent. In this paper, the Raman spectrum of veterinary drug adulteration is detected through experiments, and the optical system of Raman spectrometer is designed for the detection of veterinary drug adulteration. The main contents include: (1) in this paper, the Raman spectrum analysis method of adulteration of veterinary drugs is studied by combining Raman spectroscopy with chemometrics. The principle of qualitative discrimination and quantitative analysis of partial least square method is introduced. Then penicillin, a common drug in veterinary medicine, is used as the research object, based on partial least square method. The penicillin samples doped with glucose and starch were qualitatively distinguished, and the content of penicillin in adulterated drugs was quantitatively analyzed. According to the demand of veterinary drug detection, this paper is based on the requirements of veterinary drug detection, such as the elimination of singular samples and the effects of various preprocessing methods and their combinations on the model. The external optical path system of the portable Raman spectrometer is designed. The external optical path system consists of two parts: laser excitation and scattering light collection. The excitation path matches the numerical aperture of the laser output fiber. With a magnification of 1: 1, the system's plot size is the same as that of the Alli spot, and it has a strong focusing power. The scattering light collection path has a numerical aperture of 0.44, which enables the Raman scattering light at a larger angle to enter the system. The root-mean-square radii of zero field of view and 50 渭 m field of view are 3.949 渭 m and 5.074 渭 m, respectively. The diffraction characteristics of volume holographic grating are analyzed, the optical system of transmission Raman spectrometer is designed, the length of the system is less than 10 cm, the numerical aperture of object is 0.22, and the resolution is 0.6 nm in the range of 790-1014 nm.
【学位授予单位】：中国科学院研究生院(长春光学精密机械与物理研究所)
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S859.79

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